제주도에서 푸사리움가지마름병의 자연감염목으로 추정되는 해송에서 분리된 균을 분자생물학적 기법을 이용하여 기존의 푸사리움가지마름병균과 비교하고, 분리된 균을 리기다소나무× 수식 이미지리기테다소나무 2, 4년생과 해송 2, 3, 6년생 묘목에 인공 접종하여 수종 및 수령별 감수성을 파악하고자 본 실험을 수행하였다. Site 1(FT)에서는 총 샘플 14 개체 중 13 개체에서 푸사리움균이 분리되었으며, Site 2(FS)에서는 9 개체 중 7

Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. × rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. × rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. × rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. × rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. × rigitaeda.