뻐ny studìes have shown the anti-proli ferative effects of irondeprivation on cancer cell s‘ but the effects 01' iron-chelators on oral cancer have not been clearly elucidated , To investigate the effects of an iron chelato r, desferrioxamine( 01"O).on the growth of ilIllTIortali zed human o1'al ke ratinocytes(IHOK), primary oraJ cancer cel ls(HN4)‘ metastatic oral cancer cell s(HN12) , and human skin keratìnocytes(HaCaT) in the MTr assay, three-dimensional(3D) raft cul tmes, Western blott ing, cell cycJ e analysis‘ nuclear staining‘ and cytochrome c expression for apoptosis s ig naling pathway were used OFO inhibited the growth of immortalized IHOK and HaCaT and mal ignant HN4 and HN12 keratinocytes in a time- and dose-dependent manner according to the MTT assay, The 3D organotypic cu l tu re also revealed that OF'O-treated cells showed less epithelial maturation, less surface keratinizati on‘ and de creased epithelia l thickness, The major mechanìsm of growth inhìbition with the micromolar 0 1"0 treatment was by the induction of apoptosis‘ which was supported by nuclear OAPI staining, ONA fragmentation analysis, and J10w cytometric analysis for sub-Gl phase ar rest and Annexin V-1"ITC stainìng, Furthermore‘ Bax expression in creased together with p53 and p21WAF1!CIPl, whìle the Bcl-2 expression decreased in the immortalized and malig nant keratinocytes treated with 01"0 , Time-dependent cytochrome c from mitochondria was observed in D1"O-treated [l-IOK and 0 1'머 cancel‘ ceJJ s, and was accompanied by the activation of caspase-3 in IHOK cells. These resu lts demonstrate that 0 1"0 has growth inhibitory effects on immortalized and malignant oral keratinocyLes Lhrough the induction of apoptosis and suggest that further evaluation of OFO as a potcntial thcrapcutic agent for human oral precancerous and cancerous lesions is warranted

The purpose of this paper is to evaluate the noise level and source, reduce the subway lineⅡ noise. As a result of measurement of subway line Ⅱ in Busan the highest value section of uproad line was from Jigegol to Motgol by 89 dB(A). The sections of conversation and listening interference(over 80 dB(A)) were 21 sections(55%) of 38 sections. Among these sections, 15 sections(71%) were produced rolling noise, 3 sections(14%) squeal noise, 2 sections(10%) braking noise and 1 section(5%) fan noise, and then a main noise source was the rolling noise. In case of downroad line, the highest value section was from Busan Metro Art Museum to Centum city, Motgol to Jigelgol by 88 dB(A). The sections of conversation and listening interference(over 80 dB(A)) were 18 sections(47%) of 38 sections. Among these sections, 15 sections(83%) produced rolling noise, 2 sections(11%) squeal noise and 1 section(6%) braking noise were investigated in this study. and then a main noise source was the rolling noise.