This study was conducted to investigate the effects of type of the sugar supplemented to the extender on the vigor, viability and intact acrosomal rates of frozen-thawed dog spermatozoa. The ejaculated semen was diluted with TRIS-citric acid extender containing 200mM TRIS, 73mM citric acid, 6% (v/v) glycerol, 20% (v/v) egg yolk, 1% (v/v) antibiotics (streptomycin/penicillin), 44 mM sugar, which was either glucose, fructose or glucose-fructose combination, and distilled water to make the final volume of 100ml. Extended semen samples were cooled at for an hour, packaged in 0.25ml straws, equilibrated for 10 minutes in liquid nitrogen vapor, and frozen in liquid nitrogen. Samples were thawed by placing straws into water for 120 seconds. After thawing, vigor, viability and intact acrosomal rates of frozen-thawed semen were compared according to type of sugar. No significant differences were observed between glucose and fructose groups. In addition, combination of the 2 sugars also did not show any significant differences in the vigor, viability and intact acrosomal rates. In conclusion, glucose and fructose were equally efficient as sugar supplements for freezing extender.

In Namyang Bay of western Korea, macrotidal-flat deposits are divisible into three late Quaternary units: Unit M1 of upper marine mud, Unit T1 of middle siderite-bearing terrestrial clay, and Unit M2 of lower marine mud. Unit M1 represents typical Holocene intertidal mudflat deposits, showing a coarsening-upward textural trend. It probably resulted from the continual retrogradation of tidal flat during the mid-to-late Holocene sea-level rise. Reddish brown-color Unit T1 consists of homogeneous clay with abundant freshwater siderite grains and plant remains. Unit T1 is clearly separated from the overlying Unit M1 by a sharp lithologic boundary. Radiocarbon age, siderite grains and lithologic features indicate that Unit T1 is originated from freshwater bog or swamp deposition infilling the localized topographic lows during the early Holocene age. Overlain unconformably by early Holocene swamp clay, Unit M2 is orange to yellow in color and mottled, suggesting significant degree of weathering during the sea-level lowstand. Such subaerial oxidation is confirmed in the vertical profiles of geotechnical properties, clay mineral assemblages and magnetic susceptibility. Unit M2 appears to be correlated with the upper part of the late Pleistocene tidal deposits developed along the western Korean coast. The sedimentary succession of the Namyang-Bay tidal-flat deposit provides stratigraphic information for the Holocene-late Pleistocene unconformity and also permits an assessment of the preservation potential of the late Pleistocene marginal marine deposit along the western coast of Korea.