This study was conducted to investigate the effects of type of the sugar supplemented to the extender on the vigor, viability and intact acrosomal rates of frozen-thawed dog spermatozoa. The ejaculated semen was diluted with TRIS-citric acid extender containing 200mM TRIS, 73mM citric acid, 6% (v/v) glycerol, 20% (v/v) egg yolk, 1% (v/v) antibiotics (streptomycin/penicillin), 44 mM sugar, which was either glucose, fructose or glucose-fructose combination, and distilled water to make the final volume of 100ml. Extended semen samples were cooled at for an hour, packaged in 0.25ml straws, equilibrated for 10 minutes in liquid nitrogen vapor, and frozen in liquid nitrogen. Samples were thawed by placing straws into water for 120 seconds. After thawing, vigor, viability and intact acrosomal rates of frozen-thawed semen were compared according to type of sugar. No significant differences were observed between glucose and fructose groups. In addition, combination of the 2 sugars also did not show any significant differences in the vigor, viability and intact acrosomal rates. In conclusion, glucose and fructose were equally efficient as sugar supplements for freezing extender.

In this study, growth of pepper seedlings was testedby treating organic materials in a plastic green house in Ssangok-ri Dogok-myeon, Hwasun-gun, Jeollanam Province from February 3, 2010 to April 19, 2010. The raised seedlings were planted to the organic planting area with an interval of 70cmx55cm on April 20 in Deogam-ri Gogeum-myeon Wando-gun and red peppers were harvested at 7 times from July 22 to September 29. Before planting, the plant length of the overgrowth of seedlings was reduced by 26.8% using plant powder treatment as compared to the control group. The red pepper yield was 27% higher than in the control group when 20g of bone ash powder and 20g of plant powder were mixed. The yield tended to increase in other treatments compared to the control group.