This study was conducted to investigate the effects of type of the sugar supplemented to the extender on the vigor, viability and intact acrosomal rates of frozen-thawed dog spermatozoa. The ejaculated semen was diluted with TRIS-citric acid extender containing 200mM TRIS, 73mM citric acid, 6% (v/v) glycerol, 20% (v/v) egg yolk, 1% (v/v) antibiotics (streptomycin/penicillin), 44 mM sugar, which was either glucose, fructose or glucose-fructose combination, and distilled water to make the final volume of 100ml. Extended semen samples were cooled at for an hour, packaged in 0.25ml straws, equilibrated for 10 minutes in liquid nitrogen vapor, and frozen in liquid nitrogen. Samples were thawed by placing straws into water for 120 seconds. After thawing, vigor, viability and intact acrosomal rates of frozen-thawed semen were compared according to type of sugar. No significant differences were observed between glucose and fructose groups. In addition, combination of the 2 sugars also did not show any significant differences in the vigor, viability and intact acrosomal rates. In conclusion, glucose and fructose were equally efficient as sugar supplements for freezing extender.
In Namyang Bay of western Korea, macrotidal-flat deposits are divisible into three late Quaternary units: Unit M1 of upper marine mud, Unit T1 of middle siderite-bearing terrestrial clay, and Unit M2 of lower marine mud. Unit M1 represents typical Holocene intertidal mudflat deposits, showing a coarsening-upward textural trend. It probably resulted from the continual retrogradation of tidal flat during the mid-to-late Holocene sea-level rise. Reddish brown-color Unit T1 consists of homogeneous clay with abundant freshwater siderite grains and plant remains. Unit T1 is clearly separated from the overlying Unit M1 by a sharp lithologic boundary. Radiocarbon age, siderite grains and lithologic features indicate that Unit T1 is originated from freshwater bog or swamp deposition infilling the localized topographic lows during the early Holocene age. Overlain unconformably by early Holocene swamp clay, Unit M2 is orange to yellow in color and mottled, suggesting significant degree of weathering during the sea-level lowstand. Such subaerial oxidation is confirmed in the vertical profiles of geotechnical properties, clay mineral assemblages and magnetic susceptibility. Unit M2 appears to be correlated with the upper part of the late Pleistocene tidal deposits developed along the western Korean coast. The sedimentary succession of the Namyang-Bay tidal-flat deposit provides stratigraphic information for the Holocene-late Pleistocene unconformity and also permits an assessment of the preservation potential of the late Pleistocene marginal marine deposit along the western coast of Korea.
When paprika is cultivated during summer season, thrips starts to occur in the early growing season and reaches its peak in late June. In the treatment using Orius laevigatus and Neoseiulus cucumeris, natural enemies to thrips, thrips density was 87.6/trap and 51.31trap on June 23. After Orius laevigatus and Neoseiulus cucumeris were treated twice on June 24 and July 23, the number decreased considerably. On August 23, it was 36.9/trap and 40.3/trap and from the mid August to early September thrips density remained low. Effect of Orius laevigatus and Neoseiulus cucumeris on suppression of thrips density was 35.7% and 44.6% on July 22, however, the number went up to 88.8% and 80.5% on September 3.
Investigation on oriental melon was carried out for 30 minutes starting at 7 PM every day from March 21 to May 24 to find out the effect of light emitting diode on seedling quality, grafting, growth and flowering of oriental melon. According to the result of the investigation, plant height was longer in Blue, Infrared, Red+Blue and Red treatment and leaf number was higher in Blue, Red+Blue and Infrared treatment than those of control. No big difference was identified between control and Yellow, Green, Ultraviolet treatments. Grafting rate was high in Green, Red+Blue and Green treatment. The number of flower evef}' week in control was nine, the number was almost 1 higher in White and Ultraviolet A treatments, but it was 1 to 4 lower in the rest of treatments. The number of female flowers of control was 10, however, it was 21 in Infrared treatment, 17 in White, 15 in Ultraviolet, 13 in Red+lnfrared, 12 in Blue and Red+Blue, 11 in Yellow and 8 in Green.