본 연구는 장기요양 기관 종사자들의 감염관리에 대한 인지도와 수행도 현황조사를 통해 감염 관리 실태를 파악하여 장기요양 기관의 종사자를 위한 효율적인 감염관리 및 교육 프로그램 개선을 위해 수행되었다. 연구 대상자는 C 지역의 장기요양 기관 35곳의 종사자 134명이며 2021년 7월 12일부터 7월 30일까지 구조화된 설문지를 사용하여 자료를 수집하였으며 SPSS 22.0 program을 이용하여 자료 분석하 였다. 연구 결과 대상자의 감염관리 인지도에 영향을 미치는 요인은 종사자의 직종(F=3.181, p=.032), 감 염관리 교육 경험(F=6.372, p=.013) 이었으며 감염관리 수행도 또한 종사자의 직종(F=3.972, p=.010), 감 염관리 교육 경험(F=4.403, p=.038)이 영향을 크게 미쳤다. 대상자의 감염관리 인지도와 수행도는 양의 상 관관계가 있었다(r=.919, p<.001). 본 연구의 결과를 종합해 볼 때, 코로나-19 팬데믹 상황에서 장기요양 기관의 환자 안전을 위해서는 종사자가 감염관리 중요성을 인지하는 것뿐만 아니라 직접 감염관리 행위를 수행하는 것이 매우 중요하다. 이를 위해서는 기관의 규모에 상관없이 직종별, 교육 주제별 체계적이고 지 속적인 교육과정 마련과 감염관리체계의 질적 향상을 위한 평가 시스템의 법적 강화가 필요할 것으로 생각 한다.

Selecting an appropriate antigen with optimal immunogenicity and physicochemical properties is a pivotal factor to develop a protein based subunit vaccine. Despite rapid progress in modern molecular cloning and recombinant protein technology, there remains a huge challenge for purifying and using protein antigens rich in hydrophobic domains, such as membrane associated proteins. To overcome current limitations using hydrophobic proteins as vaccine antigens, we adopted in silico analyses which included bioinformatic prediction and sequence-based protein 3D structure modeling, to develop a novel periodontitis subunit vaccine against the outer membrane protein FomA of Fusobacterium nucleatum. To generate an optimal antigen candidate, we predicted hydrophilicity and B cell epitope parameter by querying to web-based databases, and designed a truncated FomA (tFomA) candidate with better solubility and preserved B cell epitopes. The truncated recombinant protein was engineered to expose epitopes on the surface through simulating amino acid sequence-based 3D folding in aqueous environment. The recombinant tFomA was further expressed and purified, and its immunological properties were evaluated. In the mice intranasal vaccination study, tFomA significantly induced antigen-specific IgG and sIgA responses in both systemic and oral-mucosal compartments, respectively. Our results testify that intelligent in silico designing of antigens provide amenable vaccine epitopes from hard-to-manufacture hydrophobic domain rich microbial antigens.

This study was conducted to investigate the changes of hormone levels of serum progesterone (P4) and estradiol-17 β (E2) in sows of Landrace (L), Yorkshire (Y) and F1 (L × Y) (respectively n=3) with excellent ability, and to provide a baseline data for improving reproductive performance. In this experiment, the sows at the age of 12 months or more were used. The sows were fed by two way methods, one is conventional methods and the other is 3 days-flushing feed before estrus. Each pig’s blood was collected in 3, 6, 9, 12, 15 and 18 days after the estrus for the analyses of P4 and E2. Serum was separated by centrifugation for 15 min. with 3,000 rpm. Progesterone and estradiol-17β were measured by immunochemical assay (ELIZA test). In conventional feeding, serum progesterone levels were significantly (p<0.01) higher in F1 than in L and Y. No significant differences in P4 concentrations were seen between the L and Y of sows. Serum E2 levels were similar the serum progesterone levels. In the case of flushing feed, the tendency of hormonal changes were similar to conventional methods. But almost of hormonal levels were a little higher than that of conventional methods. P4 level of L and Y in flushing feed were significantly different (p<0.01). Serum E2 level of Y in flushing feed was significantly different among the breeds (p<0.01). These results were similar to the tendency of hormonal changes in general sows and moreover, flushing feed is known to develop the swine production, these results proved the fact of the methods. And these results suggested that more studies about hormonal changes in sows according to seasonal and nutritional factors should be needed.