Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.

경주 방폐장은 궁극적으로 80만 드럼의 폐기물을 수용하는 처분시설이다. 대부분 국외 처분시설의 경우도 정확히 완충의 의미가 아니더라도 관리의 정도를 차별하기 위하여 구역을 나누어 관리하는 것이 일반적이다. 국내 처분시설에서의 완충공간 설정에 대한 규제요건은 운영 중에는 원자력발전소와 크게 다르지 않아 운영중 정상운영 및 사고시 처분시설 제한구역 경계에서의 설계목표치나 성능목표치의 만족여부가 가장 주요한 요건이 된다. 폐쇄 후에의 완충공간의 의미는 제도적 관리기간 중에 부주의한 침입자가 침입하는 행위를 방지하는 최소한의 영역으로 설정될 수 있다. 부주의한 침입행위 중 직접적으로 피폭을 유발할 가능성이 가장 높은 우물이용 시나리오에 대한 안전성 평가결과가 운영중 평가결과를 바탕으로 설정된 완충공간에 적용하여도 충분히 성능목표치 만족함을 보임으로써 적합성을 확인한다. 현재 본격적인 건설을 앞둔 경주 처분시설의 완충공간 설정에도 동일한 절차와 개념이 적용되었고 규제요건과 방사선방어적으로 만족하는 구간이 설정되었다. 단, 처분시설의 활용면적은 향후 수십년간 점차로 증가하면서 그 형태가 변하게 될 것이다. 처분시설의 처분방식이나 처분용량이 달라지게 되면 10만 드림의 처분을 기준으로 설정한 제한구역이나 완충공간은 향후 변동될 것이 확실함에 따라 이에 대한 고려도 추후 반드시 필요하다.

Samchunpo(Sin Hyang) Harbor is located in the bay of Sa Chun, the central south coast of Korean peninsula. The harbor and coastal boundaries have been protecting by natural coastal islands and shoals. Currently, The Sin Hyang harbor needs maintenance and renovation of the sheltered structures against the weather deterioration and typhoon damages. Consequently to support this, the calculation of accurate design wave through the typhoon wave attack is necessary. In this study, calculation of incident wave condition is simulated using steady state spectrum energy wave model(wide area wave model) from 50 years return wave condition. And this simulation results in wide offshore area were used for the input of the extended mild slope wave model at the narrow coastal area. Finally, the calculation of design wave at Sin Hyang harbor entrance was induced by Boussinesq wave model(detail area wave model) simulation. The numerical model system was able to simulate wave transformations from generation scale to shoreline or harbor impact. We hope these results will be helpful to the engineers doing placement, design, orientation, and evaluation of a wide range of potential solutions in this area.

Paeoniae radix has been widely used for its anti-allergic, anti-inflammatory and analgesic effects, and demonstrated to have anticonvulsant, memory enhancing and anxiolytic activities. The present study was performed to examine the protective effect of methanol extract of Paeoniae radix (PR) from Paeoniae Japonica Miyabe et Takeda (Paeoniaceae) on hydrogen peroxide (H2O2)-induced neurotoxicity using cultured rat cerebral cortical neuron. H2O2 produced a concentration-dependent reduction of neuronal viability, PR, over a concentration range of 10 to 100 μg/ml showed concentration-dependent decrease of the H2O2(100 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. PR (100 μg/ml inhibited 100 μM H2O2-induced elevation of the cytosolic Ca2+ concentration ([Ca2+]c), which was measured by a fluorescent dye, flue-4 AM. PR (50 μg/ml inhibited glutamate release into medium induced by 100 μM H2O2, which was measured by HPLC, and generation of reactive oxygen species (ROS). These results suggest that PR may mitigate the H2O2-induced neurotoxiciy by interfering with the increase of [Ca2+]c, and then inhibiting glutamate release and generation of ROS in cultured neurons.