Although probiotics have been shown to improve health when consumed, recent studies have reported that they can cause unwanted side effects due to bacterial-human interactions. Therefore, the importance of prebiotics that can form beneficial microbiome in the gut has been emphasized. This study isolated and identified bacteria capable of producing biopoymer as a candidate prebiotic from traditional fermented foods. The isolated and identified strain was named WCYSK01 (Wissella sp. strain YSK01). The composition of the medium for culturing this strain was prepared by dissolving 3 g K2HPO4, 0.2 g MgSO4, 0.05 g CaCl2, 0.1 g NaCl in 1 L of distilled water. The LMBP(low molecular weight biopoymers) produced when fermentation was performed with sucrose and maltose as substrates were mainly consisted of DP3 (degree of polymer; isomaltotriose), DP4 (isomaltotetraose), DP5 (isomaltopentaose), and DP6 (isomaltoheptaose). The optimization of LMBP (low molecular weight of biopolymer) production was performed using the response surface methodology. The fermentation process temperature range of 18 to 32oC, the fermentation medium pH in the range of 5.1 to 7.9. The yield of LMBP production by the strain was found to be significantly affected by q fermentation temperature and pH. The optimal fermentation conditions were found at the normal point, and the production yield was more than 75% at pH 7.5 and temperature of 23oC.

Hydroxyapatite (HA), which is an important calcium phosphate mineral, has been applied in orthopedics, dentistry, and many other fields depending upon its morphology. HA can be synthesized with different morphologies through controlling the synthesis method and several parameters. Here, we synthesize various morphologies of HA using two simple methods: hydrothermal combustion and solution combustion. The phase purity of the synthesized HA is confirmed using X-ray diffractometry. It demonstrates that pure phased hydroxyapatite can be synthesized using both methods. The morphology of the synthesized powder is examined using scanning electron microscopy. The effects of pH and temperature on the final powder are also investigated. At 140 oC, using the hydrothermal method, nano-micro HA rods with a hexagonal crystal structure can be synthesized, whereas using solution combustion method at 600 oC, a dense cubic morphology can be synthesized, which exhibits monoclinic crystal structures.

This experiment was conducted to determine the effects of applying level of pig slurry on the characteristics of runoff and leaching water in lysimeter. Treatments were broken down into five treatments such as non-chemical fertilizer, chemical fertilizer (200㎏ N/㏊) and pig slurry (200, 400, 600㎏ N/㏊), and 3-time-repeated randomized complete block design was fulfilled. The concentrations of NO₃-N was raised(p＜0.05) in proportion to the applying level of pig slurry. NO₃-N concentration of leaching water collected from soil depth 20㎝ and 40㎝ was intensified as the applying level of pig slurry was higher (p＜0.05) in pig slurry 600㎏ N/㏊ application. In conclusion, pig slurry at the volcanic ash soil in Jeju area can replace the chemical fertilizer as it is tested that applying 200㎏ N/㏊ of pig slurry and chemical fertilizer show the same productivity of sorghum×sudangrass hybrid. But more than 200㎏ N/㏊ of pig slurry may not be appropriate because it may contaminate the runoff and leaching water even though it may increase for the forage productivity.