A combination of Polycarbonate (PC) material and Polymethylmethacrylate (PMMA), fabricated using an injection molding machine, has been investigated to determine its advantages, as studied in Ref. 1). This paper aims to investigate the optimization of PMMA/PC blend for both tensile yield strength and impact strength. Furthermore, interaction effects of process conditions on mechanical properties including tensile yield strength and impact strength of PMMA/PC blend by injection molding process are interpreted in this study. Tensile and impact specimens are designed following ASTM, type V, and are fabricated by injection molding process. The processing conditions such as melt temperature, mold temperature, packing pressure, and cooling time are applied; each factor has three levels. As a result, in comparison with optimization of separated responses, mechanical properties of PMMA/PC are found to decrease when optimizing both tensile and impact strengths simultaneously. The melt temperature is found to be the most significant interaction parameter with the mold temperature and packing pressure. In addition, there is more interaction between the mold temperature and cooling time. This investigation provides a useful understanding of the control of injection molding processing of polymer blends in optical application.

This study investigated the efficacy of four Brucella (B.) abortus recombinant proteins, namely adenylate kinase (Adk), nucleoside diphosphate kinase (Ndk), 50S ribosomal protein (L7/L12) and preprotein translocase subunit (SecB), as a combined subunit vaccine (CSV) against B. abortus infection in BALB/c mice. Immunoblotting assay showed that these four recombinant proteins as well as pcold-TF vector reacted individually with Brucella-positive serum, but not with Brucella-negative serum. The peripheral blood CD4+ T cell population was increased in CSV-immunized mice compared to PBS and pcold-TF vector groups. In addition, CSV and pcold-TF groups displayed induced IgG1 and IgG2a antibodies production compared to PBS and RB51 group, whereas IgG2a titer was higher than IgG1 titer in CSV group. The secretion profiles of IgG1 and IgG2a production together with an enhancement of CD4+ T cell population suggested that CSV did not only induce T helper 1 (Th1) T cell immunity but also humoral immunity. Therein, Th1 T cell immunity is more predominant in eliminating intracellular bacteria B. abortus. Furthermore, CSV immunization significantly reduced the bacterial burden in the spleen as well as the spleen weight in comparison to PBS and pcold-TF groups. Altogether, combination of these antigens could be potential to induce protective immunity against B. abortus infection in animals.