To concern about health increased at well-being world. It reported fault about toxic of lymphocyte cell by Chemotherapeutic agent and antibiotic agent, tolerance of cancer cell and destruction of lymphocyte and marrow cell. Study of natural medicine needed to remedy these shortcomings. In this study verified effect of anti-inflammatory and antioxidant in Ganoderma applanatum. Verify of anti-inflammatory effect checked to derive by LPS. Antioxidant effect assured by DPPH radical scavenging activity and superoxide dismutase (SOD) activity. The result of cytotoxicity by MTT-assay for anti-inflammatory effect of Ganoderma applanatum does not effect in 0.01~1mg/mL concentration. Nitric oxide (NO) inhibition effect was verified concentration dependent in all treatment groups. Inflammatory cytokine (TNF-α,IL-1β,IL-6) formation inhibition effect to derive by LPS was verified to inhibit gene revelation of inflammatory cytokine in all treatment groups. And endotoxin shock inhibition effect does not appear concentration dependent in mouse lethal test. Inflammation mediator activation inhibition effect in shock mouse liver cell decreased gene revelation in IL-1β and IL-6 at 0.5g/kg body weight group and 1.0g/kg body weight group. DPPH radical scavenging activity confirmed concentration dependent in all treatmen tgroups. SOD enzyme activity showed high activity in low concentration than vitamin C and BHT. To conclusion, Ganoderma applanatum was predicted to replace anti-inflammatory medicine and to use great antioxidant material.

Mushrooms including Mycoleptodonoides aitchisonii are used as foods and employed as folk remedies for diabetes and inflammatroy diseases. This study analyzed anti-diabetic effects of Mycoleptodonoides aitchisonii, which grows in some areas such as Gangwon-do and Jeju-do in Korea, as insulin-derived phosphorylation. When 100 ng/ml of IL-6, inflammatory cytokine was given to SK-hep1, HepG2, Akt phosphorylation by insulin was found to be remarkably reduced. In addition, metformin, Antidiabetics serving as positive control in liver was used. Mycoleptodonoides aitchisonii used for analyzing anti-diabetic effects in this test didn’t give a great impact on the decrease in Akt phosphorylation by IL-6 at high concentration. However, fruit body in Mycoleptodonoides aitchisonii inhibited the decrease in Akt phosphorylation by IL-6 according to the concentration. At the highest concentration 100㎍/ml, it had an effect of increasing Akt phosphorylation to 77%, which was decreased by 50% compared to the insulin treated group by IL-6. Therefore, Mycoleptodonoides aitchisonii fruit body used in this test activated Akt phosphorylation inhibited by IL-6 and showed a possibility of significant anti-diabetic effects compared to positive control(metformin).