This study was conducted to investigate the seasonal occurrence of Phyllocnistis citrella Stainton (Citrus leafminer; CLM) and parasitoids in Jeju region, where the damage of CLM to the citrus farm has been recently increased. For this study, annual CLM mornitoring using sex pheromone traps were conducted in the citrus farm of Jeju from 2011 to 2015. The mean annual occurrence of CLS was 477,389 and appeared to increase along the temperature.

Substrate affinity and insecticide sensitivity of acetylcholinesterase (AChE) from Daphnia magna S., Bombyx mori L., Musca domestica L., Myzus persicae S., Anguilla anguilla L., Cyprinus carpio L., Oryzias latipes T.&S., Homo sapiens L., Bos taurus L. were tested. The Km values of M. domestica AChE to acetylthiocholine (ATCh), propionylthiocholine (PTCh), butyrylthiocholine (BTCh) were 57.3 μM, 13.4 μM and 85.9 μM respectively, which were lower than those of A. anguilla, C. carpio, O. latipes, H. sapiens and B. taurus. In nontarget organisms, the I50 values of AChE to fenitroxon and DDVP were 1.5×10-6 M~7.8×10-5 M and 2.4×10-6 M~1.1×10-4 M respectively, thus they have lower sensitivity compared with M. domestica. The I50 value of M. persicae AChE to pirimicarb was 1.3×10-8 M, which was higher sensitivity compared with other test animals except D. magna. The I50 values of D. magna AChE to fenitroxon, DDVP, carbaryl, eserine, pirimicarb were 5.2×10-10 M~2.1×10-8 M, which were higher sensitivity compared with the other test animals used for this study. cDNA of Daphnia magna AChE precursor was sequenced and compared with those of Musca domestica, Drosophila melanogaster and Torpedo californica.