A gene flow has been tested from transgenic herbicide-resistant Chinese cabbage to major crops in Cruciferae. Hybridizations were made between transgenic Basta-resistant Chinese cabbage and non-transgenic Chinese cabbage, B. napus, B. juncea and Brassicoraphanus(B. campestris × Raphanus sativus, 2n=4x=38) with honey-bee mediated fertilization. Progeny populations were selected by Basta herbicide. The F1 hybrids with pat gene were again self-pollinated or cross-pollinated with nontransgenic Chinese cabbage and rape naturally or artificially. Pod setting ratio were compared as a tentative parameter for potential gene flow. Key agronomic characters were compared among the F1 hybrid populations obtained. In the days to flowering after cold treatment, the F1 hybrids of Brassicoraphanus x B. campestris and B. napus x B. campestris(GM) were the shortest (75 days), and the GM Chinese cabbage and the F1 hybrid of B. juncea x B. campestris(GM) were 87 and 93 days, respectively. In the stem length, the F1 hybrid of Brassicoraphanus x B. campestris was the longest(175 cm), and followed by the F1 hybrid of B. napus x B. campestris(GM), the F1 hybrid of B. juncea x B. campestris(GM) in order. The naturally occurring pod setting ratios were fluctuated seriously among individual and segregation lines. Natural pod setting of the transgenic Chinese cabbage was 10 to 19 %. The F1 hybrid of B. napus x B. campestris(GM) showed the ratio of 4-11%. However, the F1 hybrids of B. juncea x B. campestris and Brassicoraphanus x B. campestris(GM) did not produced seeds. The F1 hybrids with pat gene were hybridized with nontransgenic Chinese cabbage and rape by artificial bud pollination to test potential gene flow. All cross combinations except Brassicoraphanus produced seeds and the pod setting ratios ranged from 35 to 100 percentages. Thus, the potentials of the gene flow from Chinese cabbage with pat gene to the order specise in the same gene interspecies or intergeneric species and from the F1 hybrids with pat gene to nontransgenic Chinese cabbage and rape were detected. Herbicide resistant test to the acquired seeds by natural and artificial pollinations including honey-bee mediated fertilization using the same materials is going on.

In order to investigate the effect of inflow of Yangze river on the distribution of chlorophyll-a, the results of serial oceanographic observation during 2000-2005 were used. The oceanographic conditions in the northern East China Sea is influenced by the Tsushima Warm Current and low saline water derived from the Yangze river. The distributions of these water masses vary significantly by the season in the northern East China Sea. The sea surface temperature and salinity were stable and concentrations of chlorophyll-a were low in the eastern part of 126°E. On the contrary, the salinity was significantly influenced by the low saline water derived from Yangze river with the high concentrations of chlorophyll-a. It is suggested that the low saline water inflowed from the Yangze river affects high concentrations of chlorophyll-a in the northern East China Sea in summer.

Temporal and spatial variabilities of chlorophyll a (Chl-a) in the northern East China Sea (ECS) are described, using both 8-day composite images of the SeaWiFS (Sea-viewing Wide Field-of-view Sensor) and in-situ data investigated in August and September during 2000-2005. Ocean color imagery showed that Chl-a concentrations on the continental shelf within the 50 m depth in the ECS were above 10 times higher than those of the Kuroshio area throughout the year. Higher concentrations (above 5 mg/m3) of yearly mean Chl-a were observed along the western part of the shelf near the coast of China. The standard deviation also showed the characteristics of the spatial variability near 122-124°E, where the western region of the East China Sea was grater than that of the eastern region. Particularly the significant concentration of Chl-a, up to 9 mg/m3, was found at the western part of 125°E in the in-situ data of 2002. The higher Chl-a concentrations of in-situ data were consistent with low salinity waters of below 30 psu. It means that there were the close relationship between the horizontal distribution of Chl-a and low salinity water.

This study has been conducted to develop a new recycling technology of sewage sludge using a carbonization process. The carbonizing yield, the calorific value and EC(electric conductivity) of carbonized sewage sludge had a tendency to be decreased with increase of the carbonizing temperature and time, but pH and the C/N were increased with increase the carbonizing temperature and time. The whole pore volume of carbonized sludge processed in the carbonizing furnace was 0.032㎤/g, which was smaller than that in the electric furnace. But, the rates of mesopore and macropore were found to account for 100% therein. Rate of color and organic materials removal for dyeing wastewater were determined 70～97%, 78～83% on cotton yarn, 88～96%, 69～80% on wool wastewater and 77～89%, 77～87% on towel compared with powder activated carbon. Effect of carbonized sludge on chrysanthemum growth was investigated. Plant height and number of leaves was better mixture of carbonized sludge than comparison.