세리아 입자의 합성을 위하여 분무열분해 시 유기 첨가제인 EG(ethylene glycol)과 CA(citric acid)를 첨가하여 중공성 및 다공성을 갖는 CeO2 마이크로 크기의 입자를 제조하였으며 첨가량에 따른 특성을 비교하였다. 분무열분해과정, 후소성 및 볼밀링 과정을 적절히 조합하여 만든 6가지 경로에 의해 나노 크기의 세리아 입자를 합성하였다. 6가지 경로 중 EG 및 CA가 0.05M 첨가된 Ce(III)가 전구체 수용액을 이용하여 분무열분해→후소성→볼밀링→후소성의 경로에 의해 얻어진 CeO2 입자에 대해 TEM 분석으로 측정한 입자의 평균 크기 24 nm(편차=3.8 nm)는 Debye-Scherrer식에 의해 계산된 1차 입자의 크기(20 nm)와 가장 유사한 크기를 나타내었다. 제조된 나노입자분말의 형태적 및 구조적 특성을 알아보기 위하여 SEM(Scanning Electron Microscopy), XRD(X-Ray Diffractometer) 및 TEM(Transmission Electron Microscopy)을 통하여 특성을 분석하였다.

A gene flow has been tested from transgenic herbicide-resistant Chinese cabbage to major crops in Cruciferae. Hybridizations were made between transgenic Basta-resistant Chinese cabbage and non-transgenic Chinese cabbage, B. napus, B. juncea and Brassicoraphanus(B. campestris × Raphanus sativus, 2n=4x=38) with honey-bee mediated fertilization. Progeny populations were selected by Basta herbicide. The F1 hybrids with pat gene were again self-pollinated or cross-pollinated with nontransgenic Chinese cabbage and rape naturally or artificially. Pod setting ratio were compared as a tentative parameter for potential gene flow. Key agronomic characters were compared among the F1 hybrid populations obtained. In the days to flowering after cold treatment, the F1 hybrids of Brassicoraphanus x B. campestris and B. napus x B. campestris(GM) were the shortest (75 days), and the GM Chinese cabbage and the F1 hybrid of B. juncea x B. campestris(GM) were 87 and 93 days, respectively. In the stem length, the F1 hybrid of Brassicoraphanus x B. campestris was the longest(175 cm), and followed by the F1 hybrid of B. napus x B. campestris(GM), the F1 hybrid of B. juncea x B. campestris(GM) in order. The naturally occurring pod setting ratios were fluctuated seriously among individual and segregation lines. Natural pod setting of the transgenic Chinese cabbage was 10 to 19 %. The F1 hybrid of B. napus x B. campestris(GM) showed the ratio of 4-11%. However, the F1 hybrids of B. juncea x B. campestris and Brassicoraphanus x B. campestris(GM) did not produced seeds. The F1 hybrids with pat gene were hybridized with nontransgenic Chinese cabbage and rape by artificial bud pollination to test potential gene flow. All cross combinations except Brassicoraphanus produced seeds and the pod setting ratios ranged from 35 to 100 percentages. Thus, the potentials of the gene flow from Chinese cabbage with pat gene to the order specise in the same gene interspecies or intergeneric species and from the F1 hybrids with pat gene to nontransgenic Chinese cabbage and rape were detected. Herbicide resistant test to the acquired seeds by natural and artificial pollinations including honey-bee mediated fertilization using the same materials is going on.