This study analyzed the effect on weight and average daily gain, carcass grade, and blood characteristics and immunity in case of dietary addition of pegmatite to Hanwoo steers. Relative to Hanwoo steers, initial body weight was 257.3±11.74 kg (8-months old after birth). Forty-eight Hanwoo steers were fed for 24 months using random arrangement (4 treatment plots×4 heads×3 repeat). The treatment plot (498.75~500.82 kg) had higher weight during the early fattening stage compared with that of the control plot (474.37 kg) (p<0.05). Regarding total cholesterol of the blood, the value was similar to one during initiation of testing, but the value increased gradually based on the progress of fattening. In addition, the triglyceride was considerably low in T2~T3 plots with high addition of pegmatite during the late fattening stage compared with other treatment plots (p<0.05). This study revealed a tendency in the IgG content that represented immunity as the addition of pegmatite became higher (p>0.05). This study found that the treatment plot (441.55~452.10 kg) had higher carcass weight compared with the control plot (436.30 kg). However, this study revealed that the treatment plot (2.30~2.55 points) had higher carcass quantity grade compared with the control plot (2.11 points). This study found that the control plot (3.55 points) had higher carcass quality grade compared with the treatment plot (2.67~3.09 points) (p>0.05). With studies combined, it was determined that dietary addition of pegmatite to the feed as clay minerals in fattening Hanwoo steers would improve feed intake, body weight gain and carcass quantity grade.

본 연구는 보조기구 제공의 차이가 한우의 생산성에 미치는 영향을 통해 복지형 한우사육방안을 구명 함으로써 한우 복지형 사양관리의 합리적인 사육방법에 대한 기초자료를 제시하고자 실시하였다. 공시동 물은 18개월령의 한우 거세비육우 48두를 공시하여(410.63±8.76kg) 개방식 우사에서 10개월간 실시하 였다. 시험설계는 32m2의 우방에 4두씩 각각 대조구(무처리 관행구), 처리1구(개체관리용 카우브러쉬), 처리2구(놀이용 구조물), 처리3구(개체관리용 카우브러쉬 + 놀이용 구조물)로 입식하였다. 처리는 4처리 3반복으로 완전임의 배치하고 15일간 예비실험 후 수행하였다. 조사항목으로는 사양성적, 행동관찰, 혈 액성상, 도체성적 및 경제성을 각각 분석하였다. 시험결과 보조기구 제공에 따른 사양성적으로는 처리3 구에서 유의적(p<0.05)으로 우수한 경향을 나타냈고, 대조구에서 가장 낮은 사양성적을 보였다. 행동특 성 중 발현시간에서는 기립(standing)이 대조구에서 유의적(p<0.05)으로 높게 나타났으나, 섭식 (feeding), 횡와(lying) 및 걷기(walking)는 처리2구와 처리3구에서 유의적(p<0.05)으로 높게 나타났다. 빈도에 따른 행동특성에서는 음수(drinking)와 몸비비기(rubbing), 투쟁(fighting)을 제외한 모든 행동빈 도에서 유의적(p<0.05)인 차이를 보였다. 혈액성상에서는 대조구에서 백혈구와 cortisol 수치가 유의적 (p<0.05)으로 높았고, 도체성적 중 등지방두께 및 등심단면적 항목에서 대조구 < 처리1구 < 처리2구 < 처리3구 순으로 우수한 것으로 사양성적과 유사하게 동일한 패턴의 결과를 보였다. 근내지방도에서는 놀 이용 보조기구 제공에 따라 근내지방도 스코어는 향상되는 것으로 분석되었다. 또한, 출하에 따른 경제 성 분석결과로는 처리3구에서 총수익이 두당 178여만원으로 가장 높았고 대조구의 103여만원 보다 두당 75여만원의 추가 수익을 올린 것으로 분석되었다. 따라서, 본 시험결과를 바탕으로 보조기구의 제공이 한우 거세우의 사양성적, 행동발현, 혈액성상 및 도축성적에 긍정적인 경향을 미치는 것으로 판단되며, 향후 농가현장에 보급함으로써 한우산업 경쟁력 향상에 기여할 것이다.

Cryopreservation of avian semen is a useful tool to preserve genetic resource for aim of preventing extinction induced by infectious disease like avian influenza. Unlike those of mammals, data from chicken cryopreserved semen has not been showed feasible results. So, various cryoprotectants and diluents have been examined in many methods. In this report, as a major ingredient of avian seminal plasm, glutamine was substituted by alanyl glutamine to enhance physiological stability of chicken semen during freezing. We studied effect of glycerol and Dimethylacetamide(DMA) on motility and progressive motility of spermatozoa using glutamine diluent(EK-G) or alanyl glutamine diluent(EK-A) condition. The semen of Ogye was collected twice a week by the dorso-abdominimal massage method and diluted with same volume of EK-G or EK-A at 25℃ and stored for 10 min at 4℃ in cold chamber. Glycerol or DMA was added to diluted semen to reached 7% of final concentration at 4℃. After 3min of equilibration, the diluted semen was packed into 0.25ml straws and subjected to cryopreservation used freezing equipment. The packed straw were placed on height 5 cm above surface of liquid nitrogen(LN2) and held for 10min. After preserved for 2 weeks, the straw was thawed onto the 4℃ cooling bath. The images of motility and progressive motility spermatozoa were recorded by digital image recorder and analyzed by manual. The results showed 68.5% motility and 34.1% progressive motility in DMA/EKA diluent, 31.45% and 17.6% in glycerol/EKA, 45.4% and 8.6% in DMA/EKG, and 9.7% and 6.4% in glycerol/EKG. With these results, the alanyl glutamine and DMA could be used as a main composition of diluent and cryoprotectant for cryopreservation of chicken semen.

For reconstituting genetic resource(Korean Native Chicken: KNC) with grem-line chimeric chicken made with cryopreserved biastdermal cells, the experiments were carried out to optimize cryopreservating conditions. Stage X biastdemal cells were collected from KNC embryos and dissociated. Cells were susupended in medium containing cyopretectant and fetal bovine serum(FBS), and distributed into plastic ampules. Cell susupensions were seeded to induce ice formation at —7 ℃ to —35 ℃ at in the experiments, the effect of modification of dissociation way, concentration of FBS and cell density on the vaibility of frezen-thawed cells were investigated by trypan blue exclusion. Then change the way of cell dissociation from pipetting to short time vortexing, viability of frozen-thawed cell tended to be increaced from 29 % to 52 %. Increase concentraition of FBS in frozen medium from 20 % to 80 % made viability of thawed cell from 28 % to 35 %. The viability of thawed cells were 33.9% frozen at 2 embryos/ 0.5ml, and 43.6 % frozen at 20 embryos/0.5 ml. Furthermore, combination of three modifications make big improvement. The viability of frozen-thawed cell was 60 % for combinated method, and 41 % for general method. This result means the advance to practical cryoreservation of blastdermal cell of the KNC(Ogolgye breed).

This study was carried out to investigate the effects of semen on reproductive ability in crossbred Korean native chicken (KNC, 58-wk old). The body weight, volume of semen and concentration of spermatozoa, were 2.96 g, 0.40 ml, 36.58×108/ml, respectively, in KNC. The fertility and hatchability were 94.8% and 78.8% respectively in crossbred KNC. KNC(Y) was high compared to other strains in fertility. The other strains were not significantly different among 6 strains. The results of this experiment indicated that hatchability of (G) was high compared to other strains. The result of this study could be available to genetic improvement of reproductive traits as a basic reference in KNC strains. To achieve the more effective improvement of reproductive traits, addition research such as genetic parameter evaluation should be performed.