Our research is aimed at predicting recent trend and leading technology for the future and providing optimal Nano technology trend information by analyzing Nano technology trend. Under recent global market situation, Users’ needs and the technology to meet these needs are changing in real time. At this point, Nano technology also needs measures to reduce cost and enhance efficiency in order not to fall behind the times. Therefore, research like trend analysis which uses search data to satisfy both aspects is required. This research consists of four steps. We collect data and select keywords in step 1, detect trends based on frequency and create visualization in step 2, and perform analysis using data mining in step 3. This research can be used to look for changes of trend from three perspectives. This research conducted analysis on changes of trend in terms of major classification, Nano technology of 30’s, and key words which consist of relevant Nano technology. Second, it is possible to provide real-time information. Trend analysis using search data can provide information depending on the continuously changing market situation due to the real-time information which search data includes. Third, through comparative analysis it is possible to establish a useful corporate policy and strategy by apprehending the trend of the United States which has relatively advanced Nano technology. Therefore, trend analysis using search data like this research can suggest proper direction of policy which respond to market change in a real time, can be used as reference material, and can help reduce cost.

This study was conducted to compare the expression pattern of the specific factors associated with pregnancy and angiogenesis during early pregnancy in Hanwoo. Synchronized female Hanwoo (4～6 year‐old) were inseminated artificially. After 10 weeks after artificial insemination (AI), the pregnancy was tested by rectal palpation method. Three pregnant and non‐pregnant Hanwoo were used in this experiment, respectively. The plasma progesterone level was measured by ELISA. Western blot analysis was performed to detect the expression of pregnancy associated glycoprotein (PAG) or angiogenic factors (VEGF, B‐FGF, ANP‐1, and TIE‐2). The plasma P4 level was increase gradually in pregnant group and maintained high level. The concentration of PAG was significantly higher from 5th weeks in pregnant group compared to that of non‐pregnant group (p<0.05). The concentrations of the VEGF (p<0.05), B‐FGF (p<0.05), and ANP‐1 (p<0.05) were significantly increased from 6th or 7th week after AI in pregnant group, respectively. And the intensity of TIE‐2, ANP‐1 receptor, was well matched with ANP‐1 (p<0.05). Taken together, it can be postulated that the blood vessels connected with fetus and dam were formed dramatically around 40 days after AI, because the expression levels of the angiogenic factors were increased significantly from this time in pregnant Hanwoo.