The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.

본 연구는 음악과 유저의 움직임 간의 자유로운 인터랙션에 주목하는 리듬댄스 게임의 사용 자 친화적 게임 플레이 방식을 제안한다. 기존의 리듬댄스 게임들은 사용자의 움직임과 개성적 인 춤에 제한을 부여하게 되는데, 이 제한을 최소화하는 방식으로 본 연구는 댄스음악에서 가 장 기본 토대를 이루는 비트값을 사용하는 게임 방식을 선택하였다. 음악의 비트를 느끼는 방 식은 어깨, 무릎, 고개의 세부분으로 나누어 키넥트를 통해 추출된 각 신체 포지션 값으로 자동 적으로 파악된다. 이를 통해 사용자는 게임의 정해진 규칙이 아닌 자신의 춤을 추는 개인적 방 식으로 게임을 즐길 수 있게 된다.