Density of malaria vector mosquito was monitored at 24 sites in Korea to estimate malaria epidemic from 2004 to 2009. Twenty four locations include six in Gwangwon province and nine in Kyonggi province and nine in Incheon metropolitan city. Mosquitoes collected by public health centers in 24 sites were sent to each RIPHE(Research Institute of Public Health & Environment) then the RIPHE identified the malaria vector mosquitoes. KCDC analysed the results. Number of malaria vector mosquito collected in 2009 shapely decreased over 33 % when compared with number of mosquito collected from 2004 to 2009. In 2009, malaria vector mosquitoes firstly were collected at Injegun on 2nd week of April (6-13, April) and the highest peak of collected mosquito was demonstrated on 3rd week of August. Number of collected mosquito sharply decreased below 10 at 3rd week of september and None of mosquito was collected 2nd week October. Decrease of number of malaria vector mosquito collected in 2009 seem to be related with lowest temperature around midnight and precipitation days per month.

Making use of the arbitrary shock theory developed by Ulmschneider (1967, 1971) and Ulmschneider and Kalkofen (1978), we have calculated the dissipation rates of upward-travelling slow-mode acoustic shock waves in umbral chromospheres for two umbral chromosphere models, a plateau model by Avrett (1981) and a gradient model by Yun and Beebe (1984). The computed shock dissipation rates are compared with the radiative cooling rate given by Avrett (1981). The results show that the slow-mode acoustic shock waves with a period of about 20 second can heat the low umbral chromospheres travelling with a mechanical energy flux of 2.6 × 10 6 e r g / c m 2 s at a height of 300 ∼ 400 k m above the temperature minimum region.

Genetically modified (GM) crops have never been cultivated commercially in Korea, it is necessary for a thorough assessment of the risks associated with their environmental release. We determined the frequency of pollen mediated gene flow from disease resistant GM rice (OsCK1) to non-GM rice (Nagdongbyeo) and weedy rice (R55). A total of 449,711 or 164,604 seeds were collected from non-GM and weedy rice, respectively which were planted around OsCK1. Resistance of the hybrids was determined by repeated spraying of herbicide and DNA analysis using specific primer to confirm hybrids. Though non-GM rice and weedy rice have similar flowering time, the hybrids were found only in non-GM rice and out-crossing ranged from 0.018% at 0.3 m to 0.013% at 0.6 m. All of hybrids were located within 0.6 m distance from the GM rice plot in southerly direction. The meteorological factors including temperature and relative humidity during flowering time were found to be the most important factors for determining rice out-crossing. It should be considered many factors like the local weather condition and flowering time to set up the safety management policy to prevent pollen mediated gene flow between GM and conventional crop.

The β-carotene biofortified transgenic rice was developed by transforming rice cv. Nakdongbyeo with phytoene synthase (Psy) and carotene desaturase (Crt I) genes isolated from Capsicum and Pantoea. The aim of this study was to perform molecular characterization of rice transformants of T5-T7 generation harboring Psy and Ctr I genes driven by endosperm specific globulin promoter for biosafety evaluation of β-carotene biofortified transgenic rice. The structure and sequence of T-DNA in the transformation vector and the insertion sites, flanking sequences and generational stability of inserted T-DNA in transgenic rice lines were analyzed. The transformation vector consisted of right border, MAR gene, carotenogenic genes unit, herbicide resistance selectable marker unit, MAR gene and left border in sequential order. T-DNA was introduced at the position of 30,363,938-30,363,973 bp of chromosome No. 2 by adaptor-ligation PCR. Stable integration of T-DNA and stable expression of bar gene was confirmed in T5 to T7 generations. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene was not present in the genome of β-carotene biofortified transgenic rice. HPLC analysis confirmed that carotenoids were consistently detected through T5-T7 generations.

β-카로틴 강화벼 도입유전자의 도입 위치와 안정성을 도입 위치 주변염기서열 분석과 서던 분석한 결과, 벼 염색체 2번 중 30,363,938번과 30,363,973번 사이의 단일 부위에 도입유 전가 도입되었으며, T5-T7 세대 동안 도입된 모든 유전자들 이 안정적으로 유지되고 있으며, 도입유전자의 운반체인 Backbone DNA (pSB11)는 β-카로틴 강화벼에 삽입되지 않 았음을 확인하였다. 선발 마커로 도입된 PAT 단백질의 발현 분석 결과에서도 T5-T7 세대별, 생육시기별로 안정적으로 발 현됨을 검정하였으며, 목적 형질인 β-카로틴 분석 결과에서 도 모품종인 낙동벼에 비해 9.4배 함량이 증가됨을 확인하였 다. 이상의 분석기법을 통해 복수세대에서 β-카로틴 강화벼 의 도입 유전자들이 안정적으로 유지되고 목적 단백질들이 안정적으로 발현되고 있음을 확인하였다.