Honeybees (Apis mellifera) are defend on feeds for their larvae and adult bees` healthy life. The nectar is carbohydrate source for making energy. We are extract several sugars from plants for using carbohydrate source to bee. 3 sugar source was extracted from plants (watermelon, cabbage, and mandarin). Almost sample showed high level of insecticidal rate and low level feed intake rate. We were selected 2 type of cabbage sugar syrup that low level insecticidal rate and high level feed intake rate (No 6 cabbage(+fructose) was mixed with 10% fructose and No. 7 cabbage(+pollen) was made with 10 % pollen). Cabbage sugar solution has much more impurities than purified sugar. So, No.6 and No 7 sample can do up-regulation of antimicrobial genes (apideacin, defencin, abacin, and hymenopteacin). Cabbage sugar solution has much more impurities than purified sugar. Our results suggest that up-regulation of antimicrobial genes might be involved in worker through carbohydrate impurities related immune pathways.