최근 기능성 게임의 교육적인 활용에 대한 관심이 증가하면서, 게임의 교육적 효과성에 대한 많은 연구들이 이뤄지고 있다. 본 논문에서는 인지기능 향상을 위한 기능성 게임인 ‘바우바우 프렌즈’를 개발했다. 이 콘텐츠는 인지기능 중 집행기능(Executive function), 지속적 주의력 (Sustained attention), 분리적 주의력(Divided attention)을 다루고 있다. 본 연구에서 개발된 게임의 유용성을 증명하기 위해 EEG 실험을 수행했으며, 게임 전 뇌파와 게임 진행 중 뇌파를 측정하여 비교분석했다. 결과적으로 지속적 주의력 게임(Simon)과 분리적 주의력 게임(Cooking)이 인지기능 향상에 긍정적인 효과가 있는 것으로 나타났다. 보다 아동의 인지기능 향상이라는 명확한 검증을 위해서는 추후 아동을 대상으로 한 EEG 실험을 추가로 수행할 필요가 있다.

사망시간 예측은 시신의 특징만으로 정확히 측정하기 힘들다. 또한 측정 지점에 따라 곤충의 종류가 달라 다른 지역의 결과를 적용하기 어렵다. 그래서 생물체의 사체에 몰려드는 곤충, 특히 파리류의 성체와 유충을 분석하여 사망시간을 측정하고자 했다. 그래서 전라남도 나주시 오강리에서 사후에 시간에 따라 사체에 몰려드는 곤충 특히 파리류의 성체 종류와 알에서 부화한 후 유충의 발생으로 사망시간을 계산하고자 했다. 돼지 생간을 2018년 9월 5일 평균 최고 온도 21℃, 평균 최저 온도 15℃인 체육관 뒤 공터에 방치했다. 약 10시간 후 근처에 검정파리(Calliphora vomitoria), 금파리(Lucilia caesar Linnaeus), 초파리(Drosophila), 파리매(Promachus yesonicus)가 모여들었으며, 약 30시간 후 검정파리(Calliphora vomitoria)로 예상되는 알이 놓인 것을 확인하였다. 알이 부화된 후 정기적으로 유충을 채집하여 기문의 개수로 1령, 2령, 3령을 구분하였다. 이를 통해 사후시간에 따른 유충의 성장정보를 수집하였다. 정보를 바탕으로 재실험을 진행하여 하루마다 유충의 상태로 간이 놓인 시간을 측정하였다. 오차는 1~1.5일 정도로 확인하였다. 또한, 유충이 부화한 후 시기에 따라 모여드는 곤충의 종이 점차 달라져 추측한 시간의 근거가 되는 것을 확인했다.

To control pest of Brassicaceae leafy vegetable(leaf broccoli, Red Mustard Leaf, Tatsoi) which grows at vinyl house in IKSAN, Jeollabuk-do, the non-treatment was set as negative control, and treatment was divided into Parasitic natural treatment group and general treatment group. And incidence density of pest was surveyed and control effect was analyzed. As a result, in case of spring plants, the leaf damage ratio was decreased by 31% in Leaf broccoli, 30% in Red mustard leaf and 27% in Tatsoi compared to untreatment. In case of autumn plants, it was decreased by 32%, 41% and 17% respectively. The key pests were Plutella xylostella, Pieris rapae, Myus persicae Sulzer, Thrips palmi and Striped cabbage flea-beetle. Compared with the untreatment, the incidence density of each was significantly controlled. Other pests include Spodoptera exigua, Macdunnoughia purissima, Macdunnoughia purissima which showed high incidental density sometimes. In case of spring plants, the number by treatment was increased by 117% in Leaf broccoli, 85% in Red mustard leaf and 1,000% in Tatsoi. In autumn plants, it was increased by 132%, 257% and 1,077% respectively. The used Parasitic natural and eco-friendly materials were Cotesia glomerata, lacewing, ladyburg, Orius laevigatus, Encarsia formosa, Entomopathogenic nematode, Chungjigi and Togkaki. During early development of each pest, the Parasitic naturals were grazed 2~3 times at the interval of 7~10days. During the peak time, eco-friendly materials were sprayed 1~2 times. Based on this, comprehensive management model was drafted by period for each Brassicaceae vegetables pest.

To control Thrips tabaci in Korean leek and green onion which grow at vinyl house in IKSAN, Jeollabuk-do, the non-treatment was set as negative control. In general treatment group, 120 mesh gauze was installed on the side window after planting(May, 2nd) and, in spring, Orius strigicollis (1.0/m2) was grazed 3 times every 7 days from mid of May which is early development stage and In the mid of June, which is the peak stage, plant extracts were sprayed one time. In autumn, O. strigicollis was grazed 3 times every 7 days from mid of September which is early development stage, and in the mid of October, which is the peak stage, plant extracts were sprayed one time. The result shows that the leaf damage ratio was decreased by 22% in Korean leek and by 27% in green onion compared to the untreatment. And the control value of Thrips tabaci shows 78.7% in korean leak and 90.6% in green onion. The density control effect of Thrips tabaci was significantly controlled under max 6.2/plant in general treatment group compared max 25/plant in the untreatment and this result was similar in green onion. The yields by general treatment was increased by 85% in Korean leek and 56% in green onion, compared with non-treatment yields, which was 900kg/10a in Korean leek and 1,287kg/10a in green onion.

Background : Plants are the rich source of antioxidants, which plays a very important role in maintaining human health. Their antioxidant property protects cells of different organs of human beings against free radicals and free radical mediated diseases. Even though, there is lack of knowledge on the antioxidant effect of lutein present in plants. In the present study, lutein was isolated from the GreenTea leaves (Camellia sinensis) which is used as a dietary source. Methods and Results : The procedure adopted for the isolation and purification of lutein using acetone extraction and preparative high performance liquid chromatography (HPLC) is simple and less time consuming. Free radicals scavenging activity of isolated lutein from acetone extract of GreenTea was assessed by DPPH radical scavenging assay and reducing power. The isolated lutein scavenged 79% of DPPH radicals at 20 ㎍/㎖ and two fold lower concentration compared to the standard antioxidants (α-tocopherol). No significant differences were found between the reducing power of the lutein and BHT when their concentrations were high. However, significant differences were observed at relatively low concentrations, the reducing power of lutein was isolated from the GreenTea leaves was stronger than those of their acetone extract and standard antioxidants (BHA). Both electron spin resonance (ESR) and in vitro assay confirmed that lutein was isolated from the GreenTea leaves, exhibited a greater capacity for scavenging superoxide (O2 •－) and hydroxyl (OH •) radicals than standard antioxidants β-carotene and α-tocopherol respectively. Conclusion : The results proven that lutein isolated from GreenTea leaves has an efficient antioxidant ability, it could serve as an antioxidant to scavenge reactive oxygen species.

Background : Lutein, a xanthophyll, consists of chains with 8 conjugated double bounds containing closed rings on each end of the chain. This carotenoid is found in fruits and vegetables, especially dark green leafy vegetables such as green tea. In this study, we investigated the anticancer effects of purified lutein from green tea on human cancer cell lines containing prostate carcinoma cancer cells (LNCaP). Methods and Results : Prostate carcinoma cancer cells (LNCaP) were cultured and evaluated the inhibitory effect of lutein isolated from green tea compared other carotenoids (β-carotene and lycopene) on cell proliferation. Cyclin D1 and PCNA were evaluated as cell differentiation. In results, PCNA/cyclin regulates the initiation of cell proliferation by mediating DNA polymerase. Under cultural conditions, lycopene remarkably suppressed the PCNA expression prostate cancer cell line LNCaP in higher doses (20 μM - 100 μM) statistically. However, β-carotene and lutein presented the less inhibitory effects on PCNA expression. Determination of PCNA expression in control and treated cells demonstrates that lycopene did affect proliferation in LNCaP cancer cells in dose-dependent manner. However, β-carotene and lutein suppressed the cyclin D1 expression in dose-dependent manner but no in lycopene group. These results indicate that differ carotenoids presented the various suppressive ability of PCNA and cyclin D1 expression in cell proliferation. Conclusion : In conclusion, lutein suppressed the carcinogenesis of induced prostate cancer cell line by acting as a suppressor for inhibiting the expression of cyclin D.

This study aimed to evaluate the role of D-dimer in terminal cancer patients. Among 343 incurable cancer patients, only 4 patients (2.8%) had normal D-dimer levels, and the majority (n=115, 81.0%) had levels >2.0 μg/mL. The survival time was significantly differed according to D-dimer levels with a median of 48 days when D-dimer levels were <2 μg/mL and 19 days when D-dimer levels were higher. D-dimer levels were negatively correlated with survival according to Spearman’s rank correlation analysis (coefficient -0.335, p<0.001). In terminal cancer patients, D-dimer concentrations did exhibit prog-nostic significance.