The skeleton of fuel assembly is composed of top nozzle, bottom nozzle, grids, and guide tubes. In the reactor core, all the parts of the fuel assembly suffer degradations due to the condition of high temperature, pressure and water environment. Therefore, many material properties of high temperature mechanical strength, corrosion and irradiation resistance have been considered to choose the material for fuel assembly parts in the fuel development stage. The guide tubes have important roles to connect each parts and support the load of fuel assembly while the fuel is lifted. In Westinghouse 14×14 standard fuel assembly, Zircaloy-4 was used for the material of the guide tubes. Zircaloy-4 has a resistance to water corrosion and maintain good mechanical properties after the discharge from the core, so this alloy is also utilized for a fuel rod cladding material although the microstructure is slightly different due to the heat treatment difference. Thus, it is expected that there is no issue regarding the guide tube integrity after the discharge and during the storage in the pool, especially in case of low burn-up. However, the surface oxidation and resultant hydrogen pick-up can affect to the embrittlement to the Zr alloy. So, it is needed to know the actual status of spent fuel assembly by performing post-irradiation examination. In this study, the degradation level of the guide Tubes in low burn-up spent fuel assembly was investigated using the KAERI PIE facility in order to make some data which can be utilized to the baseline for evaluating the integrity of the spent fuel skeleton.