Polyploidy analysis being one of the important mechanisms to study the genetic resources and plant diversity which plays significant role in crop evolution, breeding programs, and crop improvement was conducted in this study. The root tips of nine Cymbidium genotypes were used as materials to determine their chromosome number and flow cytometric analysis of chromosomal ploidies. The chromosome number (2n=40) has been observed in all the nine genotypes. Results showed that among nine cymbidium genotypes, six were tetraploid (2n=4x=80), two diploid (2n=2x=40), and one triploid (2n=3x=60). This study provides useful chromosome landmarks and evidence about genome evolution, heteromorphic chromosomes based heterozygosity, basic chromosome number and ploidy level in the genus Cymbidium. These results can be used as theoretical base line for crossbreeding in the genus Cymbidium.