백합 종간 교잡종은 다양한 교배 방법에 의해 생산되어지 고 있다. 그러나 이들 종간 교잡종은 대부분 불임이다. 2n gametes는 백합에서 종간 교잡종 F1의 불임을 극복할 수 있 을 뿐 아니라 우수한 유전형질을 지닌 2n과의 교배에 이용할 수 있고 후대 다양한 유전형질을 기대할 수 있다. 따라서 본 실험에서는 가장 효과적으로 2n gametes를 생산할 수 있는 방법을 구축하기 위하여 백합 Oriental hybrids ‘Medusa’와 ‘Marco Polo’에 N2O를 처리하였다. 같은 Oriental 그룹 내 에서도 ‘Medusa’와 ‘Marco Polo’는 화뢰 크기별 감수분열 시기가 달랐다. 감수분열 tetrad 시기에 염색체를 관찰한 결 과 sequential spindles, tripolar spindles에 의해 2가지 다른 형태가 관찰되었다. ‘Medusa’에서 2atm의 N2O를 처리 한 화뢰 길이 10~15mm와 20~25mm에서 monad, dyad, triad, tetrad가 관찰되었다. 그러나 4atm에서는 tetrad 이외에는 검경 되지 않았다. ‘Marco polo’의 경우 화뢰 길 이 10~15mm, 25~30mm 시기에 2, 4atm의 N2O를 12, 24 시간 동안 처리한 구에서 모두 dyad, triad가 관찰되었다. ‘Medusa’, ‘Marco Polo’ 모두 dyad, triad, tetrad가 관찰 된 시기는 대조구에서 interphase와 metaphase Ⅰ에 해당 하는 시기이다. 화분의 모양은 타원형으로 처리 전·후 변화가 없었으며 처리 후 화분의 크기는 n 보다 큰 화분뿐 아니라 작 은 화분도 관찰되어 전체적으로 다양했다. 화분의 임성과 발 아율은 처리구별로 차이가 없었으나 평균적으로 처리 후가 무처리구보다 임성은 약 10%, 발아는 약 20% 정도 떨어졌다. ‘Medusa’와 ‘Marco Polo’를 이용하여 N2O를 처리한 것과 처리하지 않은 것을 정역 교배하였을 때 자방이 비대 되는 형 태가 2가지이었으나 처리 별 차이는 아니었다. 획득된 식물체 를 Flow cytometry로 검정한 결과, 무처리 ‘Marco Polo’와 2atm 24시간 32mm에 처리한 ‘Medusa’의 교배를 통해 하 나의 식물체에서 2배체와 3배체가 모두 나오는 mixoploid를 확인할 수 있었다. 그 외 실험을 통해 얻은 식물체 중에서 이 수체가 4개 발견되었다. 확실한 3배체를 가진 식물체는 관찰 할 수 없었다.

Echeveria is a genus belonging to the Crassulaceae family that comprises approximately 170 species. It is a representative plant known as a succulent with economic potential in the floriculture industry. Echeveria plants are widely distributed in dry environments and endemic to Mexico. These plants have a rosette formation and varied leaf colors and shapes, which are characteristics of interest for landscaping, cut flowers, or interior decoration. Given their range of locations in different climates or indoor conditions, it is important to have an understanding and knowledge of their leaf morphology and anatomy and how they function to provide optimum care and management. Owing to high demand in horticultural markets, many breeders have crossed their desired species. However, this method has progressively increased the number of species without proper records of parents or other natural unintended crossings, creating phylogenetic problems and identification issues. The use and understanding of phenotypes, anatomical data, and/or research to aid in taxonomic issues and improve cultural management practices have been reviewed and discussed in this paper. In this review, we have provided a brief background of Echeveria species, focusing on the challenges and studies that have attempted to address these issues.

The purpose of the study is to assess morphological and chromosomal comparison of Mini type Phalanopsis ‘KS Little Gem’ and four domestic market available cultivars (‘Queen Beer’, ‘Tony Pink’, ‘Vaviche’ and ‘Rorens’). ‘KS Little Gem’ had the highest number of leaves (14.9), while the other four types had fewer than ten. The longest leaf length was 21.5㎝ for ‘Tony Pink’, followed by 16.2㎝, 18.0㎝ and 17.5㎝ for ‘Queen Beer’, ‘Rorens’ and ‘Vaviche’ respectively. The length and width of the petals of ‘KS Little Gem’ were 29.5㎜ and 25.6㎜ respectively indicating a round shape flower compared to other cultivars. When the flower lifespan of ‘KS Little Gem’ was compared to four cultivars of the domestic market, it was found that it had a 123-day shelf life, which was twice longer than that of the four cultivars. According to chromosome analysis ‘KS Little Gem’, ‘Rorens’, ‘Tony Pink,' and ‘Vaviche’ were tetraploid (2n = 4x = 76) while ‘Queen Beer’ was diploid (2n = 2x = 38). The estimated DNA content of ‘KS Little Gem’, ‘Rorens’, ‘Tony Pink’ and ‘Vaviche’ had 4918.4, 4794.2, 4705.2 and 4964.3 Mbp respectively, which were roughly double than that of P. cornu-cervi (control, 2n = 2x = 38). However, ‘Queen Beer’, had an estimated DNA content of 2802.2Mbp, similar to that of P. cornu-cervi. The morphological features, genome size and chromosomal data reported in these studies can be used by breeders to create more efficient Phalaenopsis breeding programs.

Phalaenopsis ‘KS Little Gem’ is a new cultivar with superior ornamental qualities. Newly produced cultivars are often studied to determine the optimum growing conditions, as production management practices vary within species. We focused on the effects of temperature and fertilizer application on the growth and carbohydrate content of ‘KS Little Gem’ plantlets. Two-month-old plantlets were subjected to different temperatures (20, 25, and 30°C) and fertilizer rates (0.33, 0.5, and 1 g･L-1) inside a growth chamber. Data were collected from leaves and roots regarding their growth, fresh weight, sugar content, and starch content. The results revealed that temperature significantly affected leaves and roots parameters, fresh weight, and carbohydrate content of the plantlets. However, fertilizer rates alone showed no significant effects on the growth and carbohydrate content of plantlets. The highest leaf and root growth, root fresh weight, and leaf carbohydrate content were observed in plantlets subjected to 25°C. In addition, ‘KS Little Gem’ plants grown under 30°C had significantly inhibited growth and reduced carbohydrate content in both the leaves and roots.

Rose is one of the most economically important ornamental crops worldwide. Although rose products are widely used, limited genetic and genomic data from this species is available. Fundamental genetic knowledge can accelerate the development of superior rose germplasms. In the present study, we explored the genetic data (e.g., chromosome numbers, total chromosome length, and ploidy level) of 39 rose cultivars using conventional cytogenetic methods. Of the 39 rose cultivars tested, 36 (92.3%) were tetraploid (2n = 4x = 28). ‘Rosada,’ ‘Rosemarin,’ and ‘Hanmaum’ were diploid (2n = 2x = 14), triploid (2n = 3x =21), and tetraploid-based aneuploid (2n = 4x = 28 + 2), respectively. The total chromosome length ranged from 46.03 ± 0.55 μm in ‘Rosada’ (2x) to 138.51 ± 0.92 μm in ‘Christoper’ (4x). The chromosome information obtained in this study will be useful for rose breeding and germplasm evaluation.

Because of their attractive and colorful flowers, many species from the genus Aster serve as garden plants. Chrysanthemum owes its popularity to its ornamental and medicinal herb value. It can be used as a cut flower, potted plant, vegetable, and herbal tea. Plant breeders have attempted to identify the available species and produce new cultivars to improve the quality of chrysanthemum for commercial purposes. The use of cytogenetic studies has paved the way for identifying compatibility, ancestry, and other useful information for this undertaking. Thus, an investigation was conducted into the chromosome numbers of 23 wild Asteraceae species in Republic of Korea to determine their genetic characteristics and variations. The somatic chromosome spread has been used for chromosome counting. The results revealed that Asteraceae species have a chromosome range from 18 (diploid) to 54 (hexaploid). These findings provide primary and important information on the chromosome numbers in chrysanthemum plants that can be used to select the right variety for cultivation.

Chrysanthemum is a valuable ornamental plant worldwide, and several of its species are used as herbal tea, medicinal plants, and dietary supplements, among others. Commercial cultivars have been developed through interspecific hybridization and artificial selection to improve the characteristics, production quality, and environmental adaptation for enhancing ornamental value. To better understand the recent research in cytogenetic studies of chrysanthemum, we examined studies concerning polyploidy, karyotyping, banding, fluorescence in situ hybridization (FISH) technique, and inter/intraspecific hybridization. Ploidy level is important in genomic characteristics and has a significant value to horticulturists and plant breeders. Studies have reported that flow cytometry analysis and single-dose molecular markers can be used to determine the chrysanthemum ploidy level. As for karyotyping, a better understanding of karyomorphological relationships and evolution of chrysanthemum and its closely related genera has already been gained. Moreover, karyotype parameters in chrysanthemum studies play a critical role in cultivar identification, classification, and genetic analysis. The FISH technique in chrysanthemum research provides more information on chromosome identification, sequences distribution, and evolution for expediting the development and improvement of plant species. The genomic in situ hybridization (GISH) technique can also be used to test hybridization in chrysanthemum breeding. Hence, this review of molecular cytogenetic studies of chrysanthemum will help us to have a better understanding and knowledge of the taxa breeding and the development and improvement of new cultivars.

농촌진흥청 국립원예특작과학원에서 육성된 formolongi-Asiatic(FA)종간잡종 나리 ‘Bonanza’, ‘Coral Candy’, ‘Purple Crystal’의 특성검정 및 FISH 분석을 통한 염색체 검경을 수행하였다. ‘Bonanza’, ‘Coral Candy’, ‘Purple Crystal’의 개화시기는 6월 중하순, 중순, 상순으로 각각 중만생종, 중생종, 조생종에 해당한다. 개화방향은 3품종 모두 상향이며 약간의 향기를 가지고 있다. 절화장은 101.0cm(‘Purple Crystal’)에서부터 142.3cm(‘Bonanza’)로 초장 신장성이 우수하여 절화로서의 개발이 가능하다. 화폭은 ‘Bonanza’와 ‘Coral Candy’가 17,1cm, 16.9cm로 관찰되어 대형화로 분류되며 ‘Purple Crystal’은 12.3cm으로 좁으나 화폭이 4cm 이상으로 화폭이 안정적이다. 잎의 길이는 ‘Bonanza’는 15.7cm, ‘Coral Candy’는 19.7cm, ‘Purple Crystal’은 11.1cm로 관찰되었다. 염색체 분석 결과, 3품종 모두 3배체(2n=3x=36)로 관찰되었다. FISH 분석 결과, ‘Bonanza’, ‘Coral Candy’, ‘Purple Crystal’의 5S/45S rDNA가 각각 4/11 loci, 4/12 loci, 4/11 loci로 관찰되었다. 3번 염색체를 제외하고 나머지 염색체에서 관찰되는 rDNA의 패턴이 달라 FISH 분석에 대한 결과는 품종을 구별하는 마커로 유용하게 이용 될 수 있을 것으로 기대할 수 있다.

The popularity of succulents as ornamental plants has increased in recent years. This is primarily because of their unique geometric shapes, which form a rosette, coupled with their ability to retain high levels of moisture. These features make ornamental succulents suitable as landscape plants as they can withstand extreme conditions and as potted plants for indoor spaces as they require minimal watering. Appropriate propagation techniques are important to increase production rates and plant quality in the shortest time possible. These ornamental plants may be propagated in various ways, both sexually, via seeds, and vegetatively, through a number of methods, such as stem cuttings, leaf cuttings, and micropropagation. In this review, methods for the propagation of ornamental succulents are described, including post-propagation care and management. Propagation aspects on which limited information is available are also highlighted. Potential areas for research required to produce data to further improve techniques, conservation, and rapid propagation efforts are discussed.