토마토의 착과와 수확량은 여름철 비닐하우스내 고온스트레스로 인하여 감소하게 된다. 본 연구는 여름철 화천지역에 위치한 비닐하우스에서 지베렐린(GA3)과 2,4-Dichlorophenoxy Acetic Acid(2,4-D) 처리가 토마토의 수확량, 착과율, 착과 후 과실품질에 미치는 영향을 알아보기 위해 ‘Adoration‘ 품종을 이용하여 실험하였다. GA3 0-, 5-, 10-, 15mg·L−1와 2,4-D 0-, 5-, 10mg·L−1를 개화 초기에 처리한 후 착과율, 수확량, 과실품질과 관련된 형질을 관찰한 결과, GA3 10mg·L−1처리구에서 대조구에 비해 14.2%의 착과율 증가를 보였다. GA3처리구에서는 수확량, 과중, 상품과실 수에 유의한 차이가 있었다. 2,4-D를 개화기에 처리했을 경우 착과율, 수확량, 상품과의 과중에 영향을 미쳤으며 GA3과 2,4-D를 각각 5mg·L−1 농도로 혼합처리 하였을 때 가장 높은(62.5%) 착과율을 나타내었다. 과실크기와 고형물함량은 GA3처리구에서만 유의성 있는 차이를 보였다. 따라서 여름철 비닐하우스의 고온조건에서 GA3 0mg·L−1처리구와 2,4-D 5mg·L−1를 처리했을 때 착과율이 가장 높아 많은 수확량을 기대할 수 있을 것으로 판단된다.

This study was done to characterize the anther derived regenerants (R1) including haploids and spontaneous diploids of minipaprika (cvs.‘Vine sweet-red’ ‘Vine sweet-yellow’ and ‘Vine sweet-orange’) in glasshouse. Eleven haploids (three, seven and one from red, yellow and orange, respectively) and sixteen spontaneous diploids (five, nine and two from red, yellow and orange, respectively) were grown in plastic pot with three (red, yellow and orange) anther donor (R0) F1minipaprika varieties. Regenerants were characterized for their plant and fruit characters as well as their fruit color and shape. The homozygosity of spontaneous diploid plants of each population was assessed using simple-sequence repeat (SSR) marker analysis. Haploid plants were characterized by reduced plant height, small leaves, short petiole and internode and small flower bud and all haploids showed the sterility and vice-versa in spontaneous diploid lines. The fruit biometrical traits exhibited greater variation within the spontaneous diploid plants and average value of quantitative traits is lower than standard varieties. MR-4 gave the highest yield (150.5 g) per plant followed by MY-6 (140.0 g) and MY-8 (130.5 g) and the lowest in MY-5 (31.5 g). Morphological marker such as fruit color further determinedthe microspore origin of androgenic diploids obtained in anther culture of ‘Vine sweet-red’. Of the fifteen spontaneous diploid plants, fourteen plants were identified as doubled haploids using microsatellite markers (SSR), and these homozygous lines are recommended to use in minipaprika breeding program.

Fifty-five sweet pepper (Capsicum annuum L.) germplasm from the Rural Development Administration (RDA) were analyzed for their fruit yield and fruit quality characters, including fruit weight, fruit size, pericarp thickness, total soluble content, fruit lobe, fruit shape, fruit surface and fruit color. Fruit yield per plot ranged from 200 g to 8150 g (average, 1884 g). Fruit fresh-weight per fruit ranged from 27.3 g to 200.0 g with an average of 97.2 g. Fruit length varied from 4.0 cm to 16.2 cm (average, 9.6 cm). Fruit length-to-width ratio ranged from 0.6 to 3.0 with an average 1.7. The fruit pericarp thickness ranged from 1.8 mm to 10.7 mm (average, 4.8 mm). Total soluble content was the highest (8.5˚ Brix) in K156286 and the lowest (5.3˚ Brix) in K156208 with the average value 6.7˚ Brix. Fruit lobes numbers varied from 2 to 4. Blocky, triangular, elongated, rectangular and round fruit shape identified among the sweet pepper accessions. Fruit surface varied from smooth to wrinkle and fruit color also varied. The presence of variability in fruit yield and quality traits within the sweet pepper genotypes can be utilized to develop high yielding sweet pepper variety with better fruit quality characters.

The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.

A field experiment was conducted to examine the fruit quality characters in second generation (F2) hybrid cultivar and to compare the fruit characters with original F1 hybrid cultivar of minipaprika (yellow and orange type) at the Research Farm, Hwacheon in July, 2010. Fruit characters varied within F2 population of each minipaprika type. In minipaprika yellow, fruit weight varied from 12.2 g to 50.8 g (average 28.5 g) and fruit length/width varied from 1.4 to 2.8 (average, 2.0). Pericarp thickness ranged from 1.8 mm to 4.1 mm (average, 2.9 mm). Total soluble solid (TSS) varied from 6.2˚Brix to 13.5˚Brix with an average of 8.7˚Brix. Fruit volume varied from 10.3 cc to 46.7 cc with an average of 24.4 cc. In minipaprika orange type, fruit weight ranged from 19.7 g to 42.4 g (average, 29.0 g) and fruit length/width varied from 1.5 to 2.6 (average, 2.0). Pericarp thickness varied from 2.1 mm to 4.1 mm with an average of 3.0 mm. TSS varied from 5.0˚Brix to 12.2˚Brix (average, 7.9˚Brix) and average fruit volume was 24.6 cc ranging from 10.7 cc to 35.0 cc. The average fruit quality characters in F2 population in both yellow and orange minipaprika did not differ from their F1 hybrid parent and F2 seed can be an additional way to supply high yielding hybrid cultivars at lower cost to the minipaprika growers.

Anthers of sweet pepper F1 cultivar ‘Special’ were cultured on Dumas De Vaulx (C medium), supplemented with 0.1mgL-1 2, 4-D and 0.1 mg · L-1 kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and 9.8 ºBrix, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce F1 hybrids. A total of 37 F1 hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the F1 hybrids. Superiority on yield over standard/commercial varieties were differed among F1 hybrids. Hybrid 5AVS8 x SP45 exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the F1 hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, KNU1017 x SP27, 5AVS1 x SP43, 5AVS5 x SP27, 5AVS8 x SP45, SP12 x SP38 and SP27 x SP25 hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce F1 hybrid seed for commercial production.

An unbalance of rice productions and consumptions caused serious problems in both of agricultural area and grain market of korea. In recently, various efforts for rice processing products such as rice noodles and rice wines are in progress to overcome the unstable rice market. Among them, waxy rice is predominant items in processing rice. However, varietal features of starch viscosity are not considered in the processing industries and rice breeding field. In this study, 12 waxy rices and 2 cultivars Ilmibyeo(japonica) and IR72(Indica) were studied for physicochemical and amylogram to characterize the use of waxy rices. The amylose contents of waxy rices were from 7.1 to 8.1% with soft gel consistency and relatively low alkali digestion value(1.4% KOH) compare to Ilmibyeo. In the amylogram analysis(RVU) of waxy rices, unlikely normal rices, very fast peak time(about 3.5 min.) was obtained compare to that of 6.2 min. of Ilmibyeo. And 2 to 3 groups were classified based on peak viscosity and consitency of RVU. Wangchal, Odorokimochi and Hangangchal showed relatively high peak viscosity seemed not suitable for rice cake due to the hardness speed. And most of waxy rices developed in korea were believed to be suitable for oil fried cakes(Hankwa) because of a certain grade of starch degradation speed. And Mochiminori and Midoromochi originated from japan could be a good sources in waxy rice breeding program to improve the cooking properties especially in slow down of hardness speed with very low peak viscosity, hot viscosity and cool viscosity.