After the permanent shutdown of K1 in 2017, decommissioning processes have attracted great attention. According to the current decommissioning roadmap, the dismantling of the activated components of K1 may start in 2026, following the removal of its spent fuel. Since the reactor vessel (RV) and reactor vessel internal (RVI) of K1 contain massive components and are relatively highly activated, their decommissioning process should be conducted carefully in terms of radiological and industrial safety. For achieving maximum efficiency of nuclear waste management processes for K1, we present activation analysis of the segmentation process and waste classification of the RV and RVI components of K1. For RVI, the active fuel regions and some parts of the upper and lower active regions are classified as intermediate-level waste (ILW), while other components are classified as low-level waste (LLW). Due to the RVI’s complex structure and high activation, we suggest various underwater segmentation techniques which are expected to reduce radiation exposure and generate approximately nine ILW and nineteen very low level waste (VLLW)/LLW packages. For RV, the active fuel region and other components are classified as LLW, VLLW, and clearance waste (CW). In this case, we suggest in-situ remote segmentation in air, which is expected to generate approximately forty-two VLLW/LLW packages.

Steinernematid와 heterorhabditid 선충은 쥐며느리에 침입하여 치사시킬수는 있었지만 효과는 없었다. Steinernema carpocapsae 포천 strain과 S. glaseri 동래 strain이 S. carpocapse All strain과 Heterorohabditis bacteriophora보다 효과가 있었으며, 병원성 발현에서 선충의 접종농도가 기주의 밀도보다 중요한 요인이었다.

Hepatocyte-like cells (HLCs) derived from human pluripotent stem cells have received extensive attention in the development of drug screening and toxicity testing. However, it has been reported that stem cell-derived HLCs showed hepatic functions that were too limited to be of use in drug screening and toxicity testing, possibly due to the lack of sufficient intercellular communication under conventional two-dimensional (2D) culture conditions. Therefore, a 3D differentiation system may overcome the in vitro limitation of 2D culture to produce stem cell-derived hepatocytes with mature metabolic functions. In this study, the feasibility of using a silicone-based spherofilm, specifically designed to produce spherical cell clusters, to generate uniformly sized 3D hepatic spheroids from hESCs was investigated. Hepatic spheroids generated on the spherofilm showed more homogenous size and shape than those generated in conventional low-attachment suspension culture dishes. Results of immunohistochemical analysis showed that expression of the mature hepatic marker albumin (ALB) increased over time during the hepatic maturation process. Furthermore, the 3D culture system mimicked the in vivo 3D microenvironment. Laminin, which is an important component of hepatic ECM, was expressed in hepatic spheroids. The results of immunohistochemical analysis indicated that the 3D culture environment is capable of generating an in vivo-like microenvironment. In addition, quantitative PCR analysis showed that the mature hepatic marker ALB and cytochrome P450 (CYP) enzymes CYP3A4 and CYP3A7 were expressed at higher levels in 3D culture than in 2D culture. This indicates that the 3D culture system is suitable for hepatic maturation and that our size-controlled 3D culture conditions might accelerate hepatic function. These results suggest that 3D hepatic spheroids significantly enhance metabolic maturation of hepatocytes derived from hESCs