This study aims to identify the degree of safety when mariners take their actions in several different situations. We have carried out many experiments to observe mariners' behavior and then measured the safety level based on their actions to avoid dangerous situations of ships collision. One of the most important actions that mariners have to take, either as their daily routine or when they are in a collision situation and then want to avoid that situation is the lookout. In this paper, behaviors on the lookout have been defined as a standard sequence of three steps which are "time of first detection", "time of recognition as risky vessel" and "time of starting avoiding action", and the suitability and applicability of the definition have been shown. And also we propose the risk assessment on ships collision and the recommendation for reducing ships collision at sea. Some analyzing results and the application of the results are reported. By combining these knowledge and some systematic studies, we propose the risk assessment on ships collision and the recommendation for reducing ships collision at sea.

Human eyelid adipose-derived stem cells (hEAs) and amniotic mesenchymal stem cells (hAMs) are very valuable sources for the cell therapeutics. Both types of cells have a great proliferating ability in vitro and a multipotency to differentiate into adipocytes, osteoblasts and chondrocytes. In the present study, we evaluated their stem cell characteristics after long-time cryopreservation for 6, 12 and 24 months. When frozen-thawed cells were cultivated in vitro, their cumulative cell number and doubling time were similar to freshly prepared cells. Also they expressed stem cell-related genes of SCF, NANOG, OCT4, and TERT, ectoderm-related genes of NCAM and FGF5, mesoderm/endoderm-related genes of CK18 and VIM, and immune-related genes of HLA-ABC and 2M. Following differentiation culture in appropriate culture media for 2-3 weeks, both types of cells exhibited well differentiation into adipocyte, osteoblast, and chondrocyte, as revealed by adipogenic, osteogenic or chondrogenic-specific staining and related genes, respectively. In conclusion, even after long-term storage hEAs and hAMs could maintain their stem cell characteristics, suggesting that they might be suitable for clinical application based on stem cell therapy.

The Kinematic GPS is well known to provide a quite good accuracy of positioning within an level. Although kinematic GPS assures high precision measurement on the basis of an appreciable distance between a reference station and an observational point, it has measurable distance restriction within 20 km from a reference station on land. Therefore, it is necessary to make out a simple and low-cost method to obtain accurate positioning information without distance restriction In this paper, the velocity integration method to get the precise velocity information of a ship is explained. The experimental results of Zig-zag maneuver and Williamson turn as the ship's maneuvering test, and other experimental results of ship's movement during leaving and entering the port with low speed were shown. From the experimental results, ship's course, speed and position are compared with those obtained by kinematic-GPS, velocity integration method and dead reckoning position using Gyro-compass and Doppler-log.

A new deep red colored standard rose (Rosa hybrida Hort.) cultivar, `Best Red' was developed at the Jeollanamdo Agricultural Research and Extention Services (JARES). Seedling and line selection were conducted in 2003 and its characteristics were examined three times from 2004 to 2006. 'Best Red' which was obtained by cross between 'Vital' and 'Cardinal'. It shows a red group color (RHS No. 46C), medium fragrance, and standard flower with good harmony between ray floret and flower center. The upper stem is moderate curved. The average yield during 2004-2006 was 111 stems/m2. Its vase life was 10 days. This cultivar was registered as a commercial cultivar in 2007. It is suitable for greenhouses in all regions in Korea and will be released to farmers in 2009.

A new pale pink colored standard rose (Rosa hybrida Hort.) cultivar, 'Fine Pink' was developed at the Jeollanamdo Agricultural Research and Extention Services (JARES). Seedling and line selection were conducted in 2003 and its characteristics were examined three times from 2004 to 2006. 'Fine Pink' was obtained by crosse between 'Nobless' and 'Yvessaaintlaurent'. It shows a red group color (RHS No. 56C), very weak fragrance, and standard flower with good harmony between ray floret and flower center. It has a characteristic of straight stem. The average yield during 2004-2006 was 113 stems/m2. It has 10 days vase life and resistance to powdery mildew. This cultivar was registered as a commercial cultivar in 2007. It is suitable for greenhouse in all regions in Korea and will be released to farmers in 2009.

A new light pink colored standard rose (Rosa hybrida Hort.) cultivar, 'Top Pink' was developed by the cross between 'Nobless' and 'Belami' at the Jeollanamdo Agricultural Research and Extention Services (JARES) through seedling and line selection in 2003, and characteristic evaluation during 2004-2006. 'Top Pink' shows a Red group color (RHS No. 49A), strong fragrance, and standard flower with good harmony between ray florlet and flower center, and straight stem. The yield was 131 stems/m2 averaged over three years (2004-2007). It showed 10 days vase life and was resistant to powdery mildew. This cultivar was registered as a commercial cultivar in 2007. It can be cultured in greenhouses in Korea and will be released to farmers in 2009.

A new dark pink colored standard rose (Rosa hybrida Hort.) cultivar, 'Global Pink' was developed by the cross between 'Nobless' and `Belami' at the Jeollanamdo Agricultural Research and Extention Services (JARES) through seedling and line selection in 2003 and characteristic evaluations during 2004~2006. 'Global Pink' showed Red purple group color (RHS No. 65B), very weak fragrance, and standard flower with good harmony between ray florlet and flower center. Its yield was 134 stems/m2 averaged over three years (2004-2007). It showed 10 days vase life and was resistant to powdery mildew. This cultivar was registered as a commercial cultivar in 2007. It can be cultured in greenhouses in Korea and will be released to the farmers in 2009.

로즈마리(Rosmarinus officinalis)와 라벤더(Lavandura spica L.)의 잎 절편으로부터 식물생장조절물질의 첨가에 따른 캘러스 유기율, 신초 및 뿌리의 분화율을 조사한 결과와 유기된 캘러스의 항산화 활성은 다음과 같다. 로즈마리의 캘러스 유기율은 2,4-D의 모든 처리구에서 양호 하였고, 특히 배양 90일째 2,4-D 1.0mg/L처리구에서 94%의 유기율로 가장 좋은 반응을 보였다. 로즈마리의 항산화 활성은 식물생장조절물질에 따라 다른 경향을 보였고, 2,4-D 0.1mg/L를 첨가하여 배양된 캘러스에서 43μg(RC50)으로 가장 높았으며, 2,4-D의 농도가 낮을 수 록 항산화 활성이 증가됨을 알 수 있었다. 또한 NAA, BA는 2,4-D와 반대로 농도가 증가함에 따라 항산화 활성이 증가됨을 알 수 있었다. 라벤더의 캘러스 유기는 2,4-D, NAA 및 BA의 모든 처리구에서 양호 하였으며, 로즈마리와 동일하게 2,4-D 1.0mg/L 처리구에서 98%의 유기율로 가장 양호하였다. 뿌리의 형성은 NAA 1.0mg/L을 첨가하였을 때 85%의 분화율로 가장 양호 하였다. 라벤더의 항산화 활성은 잎의 경우 69.5μg(RC50)으로 낮은 항산화 활성을 보였지만 2,4-D 0.1mg/L을 첨가하여 배양된 캘러스에서 37.6μg(RC50)으로 잎 보다 2배 정도 강한 항산화 활성을 보였으며, BA의 모든 처리구에서도 잎 보다 강한 항산화 활성을 보였다.

A new white colored spray rose (Rosa hybrida Hort.) cultivar Danmi was developed at the Jeollanamdo AgriculturalResearch and Extention Services (JARES) after seedling and line selection in 2002, and its characteristics were examined twicefrom 2004 to 2005

In order to discern the possibility of functional food product or ingredient of a new medicine, the leaf parts and fruit parts of Prunus mume was partitioned with various solvents and their antioxidative activity was measured. When the antioxidative activity of MeOH extracts of leaf parts and fruit parts of Korea and China was compared, all of them showed the highest antioxidative activity in EtOAc fraction. In case of Korean Prunus mume leaf parts showed that quantity required for RC50 to be 27.04μg in EtOAc fraction and in case of China Prunus mume leaf parts, it was 23.31 μg which is similar to that of α-tocopherol (22.14 μg) and showed the highest activation. In case of Prunus mume fruit parts MeOH extract, Korean fruit showed 29.16 μg, and Chinese fruit showed 31.21 μg in EtOAc fraction and thus Korean fruit extract showed a higher activity of antioxidant than the Chines fruit extract. When the antioxidative activity between the fruit parts and leaf parts of Prunus mume was compared, the leaf parts showed a higher antioxidative activity.

A new light pink colored standard rose (Rosa hybrida Hort.) cultivar “Temperla” was developed at the Jeollanamdo Agricultural Research and Extention Services (JARES) after seedling and line selection in 1999, and its characteristics were examined two time

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

Analysis of breeding gains in grain yield has been intensively conducted in wheat, barley, oat, maize, and soybean. Such information is limited in rice. The objective of this study was to compare the breeding gains and cultural gains contributed to yield gains of Korean rice varieties since early 1900s. Two sets of yield data were used for analysis; the historical yield data of 1908 for old japonica cultivars, and present yield data in the years from 1996 to 1998 for the six cultivars, consisting of previous two old cultivars and four contemporary cultivars. The old cultivars were two native cultivars, Jodongi and Damageum, while contemporary cultivars were two premium quality japonica cultivars, Hwaseongbyeo and Dongjinbyeo, and two Tongil-type cultivars, high yielding cultivars developed from indica/japonica hybridization, Milyang23 and Dasanbyeo. The yield differences of old cultivars between the experiments in 1908 and the experiments from 1996 to 1998 were estimated as cultural gains (1.84 tons ~textrmha-1 ) due to the improvement of cultivation technology. Yield differences between the old cultivars and contemporary cultivars were considered total yield gains during the periods. These were 2.51 tons ~textrmha-1 for japonica cultivars and 3.81 tons ~textrmha-1 for Tongil-type cultivars. From these data, the genetic gain of 0.67 tons ~textrmha-1 and 1.97 tons ~textrmha-1 were estimated for japonica cultivars and Tongil-type cultivars respectively. The ratio between cultural gain and genetic gain appeared to be 2.7:1 for japonica cultivars and 1:1 for Tongil-type cultivars. This analysis clearly showed the higher genetic contribution in Tongil-type cultivars than in japonica cultivars, suggesting a guideline to be used when planning new yield improvement programs. Additional implication has emerged when a better yield response to modem cultivation technology was found in one of the old cultivars, suggesting the combined improvement between breeding and cultural improvement is necessary for attaining the maximum yield capacity of a crop.

Kwanganbyeo is a japonica rice variety developed from the cross between Namyang 7 and SR14779-HB234-31, anelite line derived from the cross between Cheonmabyeo and Aichi 37 at the National Crop Experiment Station, RDA, and washi-tecture and light-dark gre