연구는 초분광 영상을 이용하여 오이 및 수박과 같은 박과 묘의 수분함량을 추정하기 위해 수행되었다. 오이와 수박 묘 샘플에 수분 스트레스를 가한 후 초분광 영상 취득 시스템을 이용하여 오이와 수박 묘 잎을 촬영하여 반사율을 계산하였고, 건조기를 이용하여 해당 모종의 수분함량을 측정하였다. 마지막으로 영상의 반사율과 수분함량을 이용하여 부분최소제곱회귀분석을 통해 수분함량 추정모델을 개발하였다. 오이 묘 수분함량 추 정모델은 R2 0.73, RMSE 1.45%, RE 1.58%의 성능을 보였으며, 수박 묘 수분함량 추정모델은 R2 0.66, RMSE 1.06%, RE 1.14%의 성능을 보였다. 유효범위를 넘어가는 극단치를 제거하여 모델의 성능을 다시 분석한 결과, 오이 모델의 경우 R2 0.79, RMSE 1.10%, RE 1.20으로 상승하였다. 오이와 수박 묘를 함께 분석하여 모델을 제작한 결과, R2 0.67, RMSE 1.26, RE 1.36으 로 분석되었다. 오이 모델이 수박 모델보다 비교적 높은 성능을 보였는데, 이러한 원인은 오이의 수분함량 변이가 넓게 분포되어 있었기 때문이라고 판단된다. 또한 데이터셋에서 유효범위를 넘어가는 극단치를 제거한 결과 오이 모델의 정확도 및 정밀도가 상승하였다. 결론적으로 오이 및 수박 묘 수분함량 추정모델들의 추정선의 기울기 차가 크지 않고, 서로 교차되기 때문에 두 모델 들은 모두 수분함량을 추정하는데 있어서 유의한 것으로 판단된다. 또한 샘플의 변수가 넓게 분포된 변이를 갖는다면 추정모델의 정확도와 정밀도는 분명 상승할 것이며, 개선된 모델을 이용하면 저가형 센서를 개발하는데 활용 될 수 있을 것으로 사료된다.

In order to improved use of Persicaria tinctoria seeds and to get basic information, general composition, fatty acid, mineral, amind acid analysis and physiological activity of methanol extract of Persicaria tinctoria seeds were investigated. Total calories of Persicaria tinctoria seeds were 348.00 kcal/100 g, general composition, carbohydrate, crude protein, crude fat and crude ash consisted of 7.85%, 67.90%, 10.10%, 4.00% and 10.15%, respectively. The amount of saturated and unsaturated fatty acids was showed 0.9048 g/100 g and 2,714 g/100 g, respectively. Minerals contained 100g of Persicaria tinctoria seeds were followed by K (549.5 ㎎), Mg (264.4 ㎎), Ca (216.2 ㎎), Fe (12.1 ㎎), Zn (3.0 ㎎). Total 15 kinds of amino acids were detected, these amino acids displayed higher value in the alanine (1,432.6 ㎎/100 g) and glutamic acid (1,088.8 ㎎/100 g). Total polyphenol and flavonoid contents were 11.08 ㎎/L and 3.56 ㎎/L, respectively. DPPH radical scavenging and ABTS radical scavenging activity in the methanol extract of 1,000 ㎎/L was showed 86.74% and 61.74%, respectively.

Cadmium (Cd) is one of heavy metals that can be easily absorbed by plant roots, and transported from soil to shoots. The effect of Cd concentration (0~200 μM) on the physiological responses, Cd content of shoot and root, and antioxidant enzymes by oil plants Jatropha curcas (cv. Biji Jarak) were investigated in hydroponics system for two weeks. Stomatal conductance was significantly depressed by Cd treated plants. However, the leaf chlorophyll content was unaffected by Cd treated plants. Cd contents in the roots of Jatropha were accumulated higher than those of shoots. Jatropha treated plants at 100 and 200 μM was found to accumulate more than 100 mg kg-1 of Cd in shoots. Jatropha plants had a high ablity to uptake Cd from soil and transport it from soil to shoot as a Cd hyperaccumulator plant because of plants accumulating more than 100 mg kg-1 of Cd in shoots are considered to define as Cd hyperaccumulator. These results confirm that Jatropha is a suitable candidate for the phytoremediation of high-level cadmium contaminated soils.

Brassica napus L. (Brassicaceae) plantation has increased as its seed is the primary source for bio-diesels. This study was conducted Male sterile (MS) line (cv. 'Sun-Mang') to pollen parent (cv. 'Mokpo 64') ratio of rape plants on fatty acid compositions of six sequential stages and yield performances. Ratio of MS line to pollen parent was followed, 4:2, 10:1, and 10:2, respectively. Seeds of six sequential stages (35, 40, 45, 50, 55, and 60 days after flowering) were collected and determined using SEED FAME ESTIMATION. The results showed that oleic acid content (C18:1) of the MS to pollen parent ratio (4:2) relatively increased up to 60 days after flowering compared to other ratios. Other fatty acid compositions such as palmitic acid (C16:0), stearic acid (C18:0), linoleic acid (C18:2) and linolenic acid (C18:3) contents decreased during seed maturation period. Seed weight (1000 seeds) of MS to pollen parent ratio (4:2) was higher than other treatments. These results suggest that fatty acid compositions and seed weight was affected by ratio of MS line to pollen parent. For rape seed production, a suitable ratio of MS line to pollen parent will be required to cultivate rape plantations.

Jatropha (Jatropha curcas L.) is a shrub or tree belonging the genus Euphorbiaceae and is cultivated in a tropical regions including South America, South-East Asia, India and Africa. Jatropha possibly is adapted to grow in a wide range of environments for producing non-edible oils. The objective of the study is to determine effect of salt and drought on plant growth characteristics. Treatments are thus followed, 100 and 200 mM NaCl and 5 and 10% PEG. Various physiological parameters such leaf length and width, stomatal conductance, chlorophyll fluorescence and gene expression were investigated. The results showed that from 2 days onwards, 100 and 200mM NaCl treatments were significantly suppressed leaf length, width, and stomatal conductance, but 5% and 10% PEG treatments showed a similar trend of control plants. Gene expression of JcACBP of the 100 mM NaCl was slightly decreased compred to those of other treatments. These results indicate that 100 and 200 mM NaCl treatments significantly showed negative response, but 5 and 10% PEG treatments improved plant growth and development of Jatropha.

Brassica napus L. (Brassicaceae), is one of major oilseed crops commonly cultivated cereal-growing areas after the rice harvest in Asia countries, has recently focused much attention as its seed is the primary source for bio-diesels. In rapeseed growing regions of South Korea, typical double-cropping regions are constantly or periodically received with poorly drained soil condition or encountered dried soil condition during the reproductive stage. This study was conducted on rapeseed plants subjected to both waterlogging and drought conditions for 4 days to investigate responses in physiological characteristics, fatty acid compositions and yield performances at reproductive stage. The treatments were thus as follows: (1) control normally irrigated, (2) waterlogging treatment were flooded by placing their pots inside larger plastic pots filled with tap water with a 2-cm water layer over soil surface, (3) drought treatment was not irrigated. For recovery period, rapeseed plants subjected to waterlogging for 4 days were drained after waterlogging, and irrigated daily as control. Drought treatment after recovery period was irrigated daily as control. Rate of photosynthesis and stomatal conductance of waterlogging and drought treated plants significantly decreased within 4 days. For recovery period, stomatal conductance and photosynthetic rate of the waterlogged and drought treated plants occurred level similar to that of the controls by 2 days after recovery period. Fatty acid compositions of the both waterlogging and drought treated plants did not affect, but yield performances of the waterlogging treatment significantly decreased. These results suggest that both waterlogging and drought conditions for 4 days did not influence fatty acid compositions, but affected yield performance at final harvest.

AtHMA3 is one of P1B-ATPase of Arabidopsis thaliana which transports and/or stores Cd and Pb in the plant cell. To test whether AtHMA3 can be used to develop enhanced resistance plant to Cd and Pb for phytoremediation, we overexpressed AtHMA3 in Columbia-0 using a pBI121vector containing the 35S promoter. To evaluate their Cd and Pb resistance, seeds of three different T3 homozygous(HMA3-1~3) and wild type plants(col-0) were germinated and grown on agar plates containing 50μM Cd, 500μM Pb and 50μM Cd+ 500μM Pb for 3 weeks. 35S::AtHMA3 grew better than wild type plants about 1.1-to 1.8-fold in Cd or Pb containg mediums. Fresh weights of all three lines of transgenic plants were 1.2-to 1.5-fold higher than those of wild type plants in Cd or Pb-containing medium. In addition to, we measured Cd and Pb content in both wild type and transgenic plants grown in solution with 50μM Cd, 500μM Pb and 50μM Cd+ 500μM Pb for 6 d. AtHMA3-overexpressing plants had consistently higher Cd and Pb contents than wild type plants. Chlorophyll contents were not significant difference between overlines and wild-types. These results show that HMA3 contributes to Cd and Pb resistance, and it might be useful for the phytoremediation.

Aluminum (Al) toxicity in plants is one of the major limitations to crop growth on acid soils. The Al-induced change of H+-ATPase expression has been regarded as an important mechanism for Al tolerance in soybean. To investigate whether translational regulation of plasma membrane H+-ATPase is involved in the response to Al stress, we conducted western - blot of this protein. The results show that western - blot of plasma membrane H+-ATPase in the "Sowon" (Al tolernace) significantly increased in translational expression level, while citric acid (50 μM) with Al (50 μM) treatment has not effected. In contrast, Al sensitive cultivar "Poongsannamool" inhibited expression level of plasma membrane H+-ATPase with Al treatment. Two - dimensional gel analysis were performed to determine the protein induction patterns of control and Al (50 μM, 24 h) treated soybean. There are many changes of plasma membrane proteins in both cultivars under Al stress. Especially "Sowon" was significantly enhanced the expression of the plasma membrane H+-ATPase in Al treatment. But protein expression of "Poongsannamool" was less than "Sowon". These results suggest that the regulatory role of plasma membrane H+-ATPase may involved the tolerance mechanism in soybean roots. At the present, transcriptional level of H+-ATPase is under investigation.