Seeds of some barberry species have embryo with physiological dormancy that requires a cold stratification for germination. Berberis amurensis Rupr. is a native species of Japan, Korea, the Russian far east, and parts of China. This specific plant is important for its edible fruits and rhizomes with high medicine value. This study aimed to determine the effect of stratification on germination and physiological change of B. amurensis. Seeds were placed on sterilized sand medium moistened with distilled water in 9 cm diameter petri dishes and stored at 4 and 25˚C for 0, 15, 30, 45 and 60 days. Each treatment had 40 seeds per replica, and three repetitions per treatment. Immediately after stratification, total phenolics contents (TPC) was analyzed and seeds incubated at 15/6˚C for 12 weeks. Warm stratification had a significant effect on seed forcing for germination than cold stratification treatment. At 25˚C for 60 days, stratified seed showed highest germination percentage (25.7±4.3%) and germination started in 14 days of the stored period. Whereas TPC was significantly decreased with increasing stored period. Contrastively, cold stratification had no effect on the germination ability. In the same way germination percentage of non-stratified seeds were also zero. The results confirmed that B. amurensis seeds were in a dormant state and warm stratification increased the germination ability by breaking of dormancy.

The purpose of this study was to parallel circuit training and circuit training with sonic systemic mechanism was to compare the differences in pulmonary function and chest expansion in adult men. This study was performed on 20 subjects. 20 subjects were divided into two groups; Circuit training group(n=10), Circuit training with sonic systemic mechanism(n=10). Both of the group performed the exercise 3 times a week for 5 weeks. The data was analyzed by the Repeated t-test for comparing before, during and after changes of factors in each group and the Independent t-test for comparing the between groups. The result are as follows. Circuit training group was statistically significant difference FVC, FEV1/FVC(p<.05), Circuit training with sonic systemic mechanism group was statistically significant difference PEF, VC in pulmonary function(p<.05). Circuit training group was statistically significant difference FEV1/FVC of between the two group in pulmonary function(p<.05). Circuit training group and circuit training with sonic systemic mechanism group was statistically significant difference in chest expansion(p<0.05) and there was no statistically significant difference of between the two group in chest expansion(p>.05).

We have used bulked segregant analysis to screen the strain-specific DNA marker associated thermophilic strain of Pleurotus eryngii. Bulked genomic DNAs of Pleurotus eryngii were amplified by randomly amplified polymorphic DNA (RAPD) using OP-A, OP-B, OP-L, OP-P, OP-R and OP-S primers to screen the strain-specific DNA marker. A unique DNA fragment of 550 bp was amplified with OP-S07 primer from the thermophilic strain and sequenced. A sequence characterized amplified region (SCAR) marker was designed on the basis of the determined sequence and named as OP-S07-1. The PCR analysis with the OP-S07-1 primer showed that this SCAR marker clearly distinguish the thermophilic strains from the control strains.