븐 논푼에서깐 4 차 손상유효 tensor 플 이용하여 유한 탄소성변형 에 대 한 운동학 석인 슨상해석이 소새된다‘ 이뉴 뼈대 C]L 죠 에서의 연깐:<1] 긴F 상역학의 유효응역시l 념 유 팡하여 이우어 낀다. _1: ïf ~l'_ 떤형윌-상대 에 l간 석용헬 수 었는 등샤변형원- 혹은 에나 "-]볍고}든 단L! ) 저|안 렌 운동-학적인 방맴은 유효변형윤 i’} 유한맨형융에 작용헬 수 있는 {: i{- 요 변형상태에 였어서() ) 손상탄소성멘형윤 사이의 관계쓸 zl] .강 한다 이는 산제 형상과 가상의 유효행상 .~!..유에 대한 변형장의 운 몽학 윤 직접 고 L냐 하여 수행띈 다， 이 방볍 은- 등 가변형원-이나 R셔 ik! 윤 에너지 칭우 지댄 스 i {-!:ι 변형융- 에 한정꾀 시 않으내 . 유한 변형윤에 대한 에나시등기 의 가정과 임치 함원 효여준다 본 .:.c:- f!~- 에 서 늠 걷:상 이 만{: 생영역에 서 운능 학적으 _ti~ .if_현되 l셔‘ -얀상 유 효 tenso] 는 2 차 순상 t.ensor 찰 동해 손상 f3- 유 꽁핵 석 꽉 정값이 향으모 -턱-정시워 진다

Agrobacterium을 이용한 Saccharomyces cerevisiae Acid Phosphatse 유전자 (PHO5) 의 식물체로의 도입

Triterpene saponins (ginsenosides) are main bioactive compounds in Panax ginseng. From mevalonic acid pathway, the isoprene units was synthesized that is precusor of triterpene saponin. This study provides the complete nucleotide sequence of the full-length cDNA involved in mevalonic acid biosynthesis pathway. We had a full-length cDNA sequences as followed; HMGS (1944bp, 1407bp ORF), HMGR (2120bp,1728bp ORF) and MDD (1760bp, 1263bp ORF) with partial poly (A). We were used real time PCR and High Performance Liquid Chromatography (HPLC) to analysis the expression level of the gene involved in MVA pathway and to ginsenoside contents, respectively. All cloned genes are constitutively expressed in all tested tissues but at different levels with the highest expression in fine roots. But ginsenoside contents is highest in leaf > fine root> lateral root > red berry> main root > petiol > stem. After treantment of methyl Jasmonate (MeJA), MVA pathway genes are highly expressed and ginsenoside contents was high at 72h.

Methyl jasmonate(MeJA) treatment promotes the plant secondary, especially triterpene saponin which is main compounds in Platycodon grandiflorum. We were performed 5,760 expressed sequence tags from MeJA treated hairy root. Total number of ESTs are 2,536 (811 contigs and 1,725 singletons) among them 2,299 are pass the annotation. The most abundant transcript were encoding fatty acid desaturase (FAD) and lots of secondary metabolite transcript were observed. Four MVA pathway genes (HMGS, HMGR, MK, MDD) and one MEP pathway gene (IDI) are identified. The unigene were classified into three major functional categories by standard GO (Gene Ontology) - cellular component, molecular function and biological process. In this study we were cloned four genes from ESTs : HMGS (1401bp ORF), MK (1167bp ORF), MDD (1254bp ORF), IDI (909bp ORF)

Bitter melon (Momordica charantia L.) is a tropical and subtropical vine of the family Cucurbitaceae, widely grown for edible fruit, which is among the most bitter of all vegetables. Bitter melon contains an array of biologically active plant chemicals including triterpenes, proteins, and steroids. The fruit is useful in gout, rheumatism and subacute cases of the spleen and liver diseases. It is supposed to purify blood and dissipate melancholia. It has also been shown to have hypoglycaemic properties (anti-diabetic) in animal as well as human studies. Raw materials of used in pharmaceuticals, health food, cosmetics, food, perfumery and other industries. However, bitter melon is not popular in Korea. For research, development and commercialization of bitter melon in Korea, we collected 13 varieties of bitter melon from domestic andabroad. We study biological and chemical characteristics of bitter melon. In general frui tsize and weight of Philippines and Japanese varieties was larger than that of Chinese and Korean. We are studying chemical analysis of useful compound (diosgenin and GABA) from bitter melon.