코로나 펜데믹 이전부터 한국교회는 정체되거나 축소되고 있었고, 선교사의 신규 유입은 둔화되고 있다. 세계정세는 탈세계화를 선언하며 현재의 예측이 불가능하고 미래 생존을 담보하기 어려워졌다. 이런 내외적 어려움을 극복하기 위해 지속가능한 선교를 위한 조직문화 개선과 선교사 지원 업무를 강화하는 선교사 멤버 케어를 장기적인 관점에서 바라볼 필요가 생겼다. 다음 세대 준비, 인적자원 개발, 전문가 지원, 정보교류를 강화하기 위한 도전에 직면하였다. 4차 산업 혁명 혁신의 중심에 있는 디지털 트랜스포메이션을 선교와 접목하고, 네트워크를 활용한 클라우드 기반의 상용 시스템을 구축하여 적은 자원으로 보다 많은 서비스를 제공하며, 선교사 코칭과 성장을 돕고, 선교 협업을 이루는 선교 플랫폼 구축을 제안한다. 궁극적으로, 선교 플랫폼은 1세대 선교사의 경험을 축적하여 선교사들에게 보다 빠르고 편리한 서비스를 제공하며 한국교회의 선교적 교회를 돕는 도구가 될 수 있을 것이다.

Severe maternal morbidity is frequently associated with catastrophic complications of pregnancy such as amniotic fluid embolism (AFE) or sepsis due to chorioamnionitis. The authors report a case of postpartum maternal hypoxic encephalopathy that might be attributed to AFE or sepsis due to chorioamnionitis with Escherichia coli bacteremia, and review the relevant literature. The patient presented with preterm labor, underwent Cesarean section for intrauterine fetal demise, and experienced postpartum cardiopulmonary collapse and disseminated intravascular coagulation, which led to catastrophic postpartum maternal encephalopathy.

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains 5~textrmmgL-1 kinetin, 0.5~textrmmgL-1 NAA, 2 % sucrose (or maltose), 3% sorbitol, and 500~textrmmgL-1 proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.