CYPRUS is a web-based waste disposal research comprehensive information management program developed by the Korea Atomic Energy Research Institute over three years from 2004. This program is stored as existing quality assurance documents and data, and the research results can be viewed at any time. In addition, it helps to perform all series of tasks related to the safety evaluation study of the repository in accordance with the quality assurance system. In the future, it is necessary to improve the user convenience by clarifying the relationship between FEP and scenarios and upgrading output functions such as visualization and automatic report generation. This purpose of this study is to research and develop the advanced program of CYPRUS. This study is based on building FEP, DIM and scenario databases. It is necessary to develop an algorithm to analyze and visualize the FEP, DIM and scenario relationship. This project is an integrated information processing platform for DB management and visualization considering user convenience. The first development goal is to build long-term evolutionary FEP, DIM, and scenarios as a database. The linkage by FEP item was designed in consideration of convenience by using a mixed delimiter of letters and numbers. This design provides information on detailed interactions and impacts between FEP items. Scenario data lists a series of events and characteristic change information for performance evaluation in chronological order. In addition, it includes information on FEP occurrence and mutual nutrition by period, and information on whether or not the repository performance is satisfied by item. The second development goal is to realize the relationship analysis and visualization function of FEP and scenario based on network analysis technique. Based on DIM, this function analyzes and visualizes interactions between FEPs in the same way as PID, RES, etc. In addition, this function analyzes FEP and DIM using network analysis technique and visualizes it as a diagram. The developed platform will be used to construct and visualize the FEP DB covering research results in various disposal research fields, to analyze and visualize the relationship between core FEP and scenarios, and finally to construct scenarios and calculation cases that are the evaluation target of the comprehensive performance evaluation model. In addition, it is expected to support the knowledge exchange of experts based on the FEP and scenario integrated information processing platform, and to utilize the platform itself as a part of the knowledge transfer system for knowledge preservation.

Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin present in grapes, exerts a variety of actions to reduce superoxides, prevents diabetes mellitus, and inhibits inflammation. Resveratrol acts as a chemo-preventive agent and induces apoptotic cell death in various cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is unclear. In this study, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. Resveratrol significantly accelerated mineralization as compared with the control culture in differentiation of MDPC-23 cells. Resveratrol significantly increased expression of ALP mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly accelerated expression of ColⅠmRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly increased expressions of DSPP and DMP-1 mRNAs as compared with the control in differentiation of MDPC-23 cells. Treatment of resveratrol did not significantly affect cell proliferation in MDPC-23 cells. Results suggest resveratrol facilitates odontoblast differentiation and mineralization in differentiation of MDPC-23 cells, and may have potential properties for development and clinical application of dentin regeneration materials.

촉진수송은 기존의 고분자 막에서는 힘든, 투과도와 선택도를 동시에 향상시킬 수 있는 기술 중 한 가지이다. 촉진 수송 분리막을 이용한 올레핀/파라핀 분리는 기존의 증류공정을 대체할 수 있는 기술로써 많은 관심을 받아왔다. 본 연구에서 는 테트라플루오로붕산은/질산알루미늄의 혼합염이 포함된 고분자 블렌드 기반의 촉진수송 올레핀 분리막을 제조하였다. 자유 라디칼중합법을 이용하여 폴리다이메틸실록세인-g-폴리옥시에틸렌 메타크릴레이트 가지형 공중합체를 합성하였다. 또한, 폴리 다이메틸실록세인-g-폴리옥시에틸렌 메타크릴레이트 매질에 폴리에틸렌옥사이드를 다양한 비율로 혼합하였다. 폴리에틸렌옥사 이드를 폴리다이메틸실록세인-g-폴리옥시에틸렌 메타크릴레이트 가지형 공중합체 질량 대비 70%를 혼합하였을 때, 혼합기체 선택도 및 투과도는 5.6 및 10.05 GPU에 도달하였다. 이와 같은 복합막의 올레핀 분리 성능이 향상된 이유는, 분리막에 첨가 된 은이온이 올레핀 기체분자의 선택적인 촉진 수송을 하였고, 또한 고투과성의 고분자 블렌드가 사용되었기 때문이다. 또한 은이온의 은나노입자로의 환원을 억제시키는 질산알루미늄의 첨가로 인해 복합막의 장시간 안정도를 향상시킬 수 있었다.

췌장의 신경내분비종양은 매우 드문 종양이나 최근 영상 검사가 발전하면서 진단 빈도도 증가하고 있다. 하지만 세로 토닌 분비 신경내분비 종양은 매우 서서히 진행하고 임상 양 상이 뚜렷하지 않아 진단에 어려움이 있다. 영상검사에서 폐 쇄 병변 없이 췌관 확장만 7년 동안 서서히 진행하여 췌관내 유두상 점액종양으로 의심하였던 환자에서 세로토닌 분비 신경내분비종양으로 수술 후 뒤 늦게 진단하였기에 이를 보고 하는 바이다.

Sulfur is commonly used in Asia as an herbal medicine to treat inflammation and cancer , a nd potent chemo preventi ve effects have been demons tra ted in various in vivo and in vitromodels for s ul fur-containing compounds found in natura l1y occurring product s. Here, we 1'eport the growth inhibitory and apoptosis-related effects of a n ewly developedhigh-puri ty edible sulfur(ES) on immo1'tali zed human o1'al ke1'atinocytes(IHOKs) and on oral cancer cells representing two stages of oral cancer (HN4‘ HN12) based on an 3-(4,5-Dimethylthiazol-2-yl) -2.5- diphe n yltetrazolium bromide(MTI) assay, Western blotting, cell cycle analysis, and nuclear staining. The puri ty of the ES used in thi s study was ve1'ified by high performance liquid chromatog1'aphy (HPLC) , ami no acid analysis and energy dispersive spectroscopy(EDS). ES inhibited the prolife1'ation of imrnortalized and ma lig nant o1'al kerati nocytes in a dose- and time-dependent manne1' FITC-Annex.in V staining, DNA fragmentation t esting. and Hoechst 33258 s taining revealed that ES inhibits cell growth via apoptosis. ES bl ocked cell-cycle prog1'ession at t he sub-Gl phase‘ wi th decreased expression of cyclins Dl, D2‘ and E, and their activating partn ers cdk2‘ cdk4‘ and cdkfì, and a concomitant induction of p53 and p21/WAF1. Furthe1'more, ES treatment in creased the cytosolic level of cytochrome c and resulted in caspase- 3 activation‘ and thi s effect was co1'1'elated with Bax up-regulation and Bcl-2 down-1'egulation Taken together‘ these data suggest that ES is a potential chemopreventive and chemotherapeut ic agent fo r oral ca ncer

Achondroplasia is associated with craniofacial abnormality, musculoskeletal deformations, and respiratory, cardiovascular abnormalities. So, anesthetic management is one of clinical challenges. Herein, we report and discuss a case of achondroplastic patient underwent osteosynthesis for femur fracture, which was chosen on the base of patient’s anatomical and physiological characteristics. The patient had thoracolumbar kyphosis (21.8°), previous spine surgery, short neck, obstructive sleep apnea, and mild cervical limitation. Intubation was conducted with smaller cuffed endotracheal tube under videolaryngoscope guidance. Mechanical ventilation was applied with volume control mode, using a tidal volume of 8-10 ml/kg without positive end-expiratory pressure. The patient was supine after placing support under shoulders and head during intubation and surgery. Intraoperative body temperature was maintained using air-forced waring blanket. Complete and careful preoperative evaluation is absolutely important to select the appropriate anesthetic method. Ideal anesthetic management in each case should be based on an individualized decision-making.

Background: Patent ductus arteriosus (PDA) is commonly accompanied by premature infants and associated with an increase of comorbidity and mortality. Surgery in the operation room (OR) is more familiar with an anesthesiologist, but transportation of the premature to OR has risks of hypothermia or other unexpected events. These days, PDA ligation in the neonatal intensive care unit (NICU) is highlighted because of its advantages. We compared the postoperative outcome of PDA ligation according to the operating theater in the perspective of an anesthesiologist. Methods: A total of 9 patient who underwent PDA ligation surgery from 2007 to 2018 were enrolled and divided into two groups; Group OR: surgery at the OR, Group NICU: surgery at the NICU. Data of gestational age (GA), body weight (BW), patients’ characteristics, anesthesia and hemodynamic, total hospitalization period, and outcome were collected. Results: There were no significant differences in patient characteristics (GA, BW, PDA size, and comorbidities) and the duration of anesthesia, operation and recovery. Total hospitalization period was shorter in a group OR than NICU, but there were no significant differences in postoperative outcome. Only 1 patient has expired in a group OR. Conclusions: PDA ligation surgery in NICU has the advantage to maintain the body temperature than the surgery in the OR, and there were no significant differences in the outcome of the surgery.

Extravasation is the accidental injection or leakage of fluid into the subcutaneous or perivascular tissues. Some drugs can cause serious injury such as severe tissue injury, necrosis, and etc. Here we report a case of chemical burn by sodium bicarbonate extravasation due to accidental venous puncture during arterial cannulation. A 42-years-old woman has taken emergency laparotomy surgery due to a stab wound to the abdomen. Massive blood loss has developed, and consequently vital signs were unstable and metabolic acidosis has developed. Sodium bicarbonate has administered via a peripheral intravenous line on the dorsal vein of a right hand that runs to the cephalic vein. However, the cephalic vein that runs by the side of the radial artery has punctured accidentally during the attempt of right radial artery cannulation. Second degree superficial and deep chemical burn by sodium bicarbonate extravasation has developed. Skin lesion about 3 × 4 cm2 with erythema and bullae formation has developed. There were no necrotic changes and the digital sensation was intact. Wet dressing and silicon foam dressing were prescribed. After two weeks, she was discharged. Until then, dermis exposure about 1 × 1 cm2 remained although the skin lesions became getting well.

Brugada syndrome is associated with high risk for sudden death without structural cardiac defects due to ventricular arrhythmias. A 47-years-old man with Brugada syndrome has admitted because of right patella fracture. General anesthesia with sevoflurane and remifentanil was carefully maintained according to the BIS for the maintenance of adequate anesthetic depth and to avoid tachycardia during the surgery. Blood pressure and heart rate of the patient were maintained less than 150/90 mmHg and 100 beat/min perioperatively. There were no adverse events, and the patient was discharged home after ten days.

Wolff-Parkinson-White syndrome (WPW) is a common disorder of the conduction system of the heart. Patients with such disorder may be asymptomatic or present with cardiac symptoms like palpitation and dyspnea. The anesthetic management of these patients is challenging as they are known to develop life threatening tachyarrhythmia. We report a hemodynamic management of a 53-year-old male with WPW syndrome scheduled for laparoscopic cholecystectomy under general anesthesia. We performed total intravenous anesthesia with propofol and remifentanil, and we placed the laryngeal mask airway behind the endotracheal tube using Bailey's Maneuver before extubation of endotracheal tube.

Background: We evaluated the effect of ulinastatin on paw withdrawal threshold (PWT), IL-6, and IL-10 in SD rats after spinal nerve ligation (SNL). Methods: Group C received N/S and Group E received ulinastatin IV for three days following SNL. PWT, IL-6, and IL-10 were measured on the 3rd, 5th, and 7th day. Results: Group E showed higher PWT compared to group C. IL-6 was lower in group E than in group C. No differences in IL-10 were observed between group C and group E. Conclusion: Ulinastatin increased the PWT and its effect appears to be involved with IL-6, not IL-10.

Severe maternal morbidity is frequently associated with catastrophic complications of pregnancy such as amniotic fluid embolism (AFE) or sepsis due to chorioamnionitis. The authors report a case of postpartum maternal hypoxic encephalopathy that might be attributed to AFE or sepsis due to chorioamnionitis with Escherichia coli bacteremia, and review the relevant literature. The patient presented with preterm labor, underwent Cesarean section for intrauterine fetal demise, and experienced postpartum cardiopulmonary collapse and disseminated intravascular coagulation, which led to catastrophic postpartum maternal encephalopathy.

Hepatocytes and hepatic progenitors derived from human ES cells may be a useful source for clinical application. Therefore, identification and purification of these cell types would be following important issues. There are very few candidate surface markers that can be used to identify and purify hepatic progenitor cells. In addition, indocyanine-green can be uptaken by mature hepatocytes, but cannot be applied for fluorescence activated cell sorting (FACS) due to its long emission wavelength. In the present study, we tested EpCAM as a potential marker for magnetic-activated cell sorting (MACS) of hepatic progenitors and also modified indocyanine-green into fluorescent indomonocarbocyanine for FACS-mediated sorting of mature hepatocytes after differentiation of human ES cells. Hepatic progenitor cells were sorted by MACS after incubation with anti-human EpCAM antibodies. After the final differentiation, the differentiated cells and mouse primary hepatocytes (control group) were incubated with indomonocarbocyanine and were sorted by FACS. MACS and immunocytochemistry data showed that approximately 45% of differentiated cells were EpCAM-positive cells. EpCAM-positive cells expressed α-fetoprotein, FOXa2, HnF4a, and CK18. Differentiation efficiency into albumin-positive cells was significantly higher in EpCAM-positive cells, compared to EpCAM-negative cells. Importantly, indomonocarbocyanine successfully stained cells that expressed ALB. Furthermore, FACS analysis data showed that the purity of hepatocytes that expressed albumin was significantly increased after purification of indomonocarbocyanine-positive cells. Our data demonstrated that human ES cell-derived hepatic progenitors can be efficiently isolated by MACS using EpCAM antibody. In addition, we also showed that indomonocarbocyanine can be successfully used to identify and purify mature hepatocytes using FACS.

Atrial fibrillation (AF) is a common, benign, and transient type of supraventricular arrhythmia encountered during the early postoperative period after a pulmonary resection. However, it sometimes might result in hemodynamic deterioration. We report on a case of new-onset atrial fibrillation in a 74-year-old male patient after right lower lobectomy, which was not controlled with intermittent bolus injection of esmolol and diltiazem. Successful pharmacological cardioversion was achieved with intravenous (6 mg/hr for 2 hours) followed by oral (90 mg q day) diltiazem 20 hours after this treatment. The literature on the risk factors and management of atrial fibrillation after pulmonary resection is reviewed.

Fatal pulmonary embolism occurs in 0.1% to 0.26% of cases of spine fusion surgery. Early diagnosis of pulmonary embolism is difficult, and delay in treatment can lead to a fatal outcome. We report on a case of symptomatic pulmonary embolism which developed after induction of general anesthesia in a patient who had been immobile because of a previous spinal operation one week prior to this. Despite a negative preoperative ultrasound examination for deep vein thrombosis, the patient had developed a pulmonary embolism, which was diagnosed using intraoperative transesophageal echocardiography. Despite intensive cardiopulmonary resuscitation and thrombolytic therapy, the patient died within a few hours of diagnosis.

Cervical epidural injection is a common treatment for management of neck, shoulder, and upper extremity pain. Although several complications are possible, cardiopulmonary arrest and intraventricular pneumocephalus are rare serious complications, mainly occurring when the dura has been punctured. We report on a case of cardiopulmonary arrest and intraventricular pneumocephalus after cervical epidural injection with loss of resistance technique using air. After performance of cardiopulmonary resuscitation for 30 minutes, a pulse was regained; however, the patient’s mental status was coma. Despite receiving conservative treatment, the patient ultimately died without recovery of consciousness.

Central venous catheterization can induce cardiac arrhythmias, such as, premature atrial or ventricular complexes, which are typically transient events. However, sometimes it initiates supraventricular tachycardia (SVT) with hemodynamic repercussions. We present a case of supraventricular tachycardia with cardiovascular collapse during central venous catheterization in a 32-year-old woman with an ovarian mass who required exploratory laparotomy. At first, the guide wire was withdrawn and carotid sinus massage attempted, but the patient did not return to sinus rhythm. Eventually, reversion to sinus rhythm was achieved after injecting adenosine.