We attempted to control the maturation promoting factor (MPF) activity and investigated the subsequent reprogramming of bovine somatic cell nuclear transfer (SCNT) embryos. Serum‐starved adult skin fibroblasts were fused to enucleated oocytes treated with 2.5 mM caffeine or 150 μM roscovitine. The MPF activity, nuclear remodeling patterns, chromosome constitutions and development of SCNT embryos were evaluated. Methylated DNA of embryos was detected at various developmental stages. The MPF activity was increased by caffeine treatment or reduced by roscovitine treatment (p<0.05). Blastocyst development was higher in the caffeine‐treated groups (27.6%) than that of the roscovitine‐treated group (8.3%, p<0.05). There was no difference in the apoptotic cell index among the three groups. However, the mean cell number of blastocysts was increased in the caffeine‐treated group (p<0.05). Higher methylation levels were observed in the Day 3 embryos of the roscovitine‐treated group (50.8%), whereas lower methylation levels were noted at Day 5 in the caffeine‐treated group (12.5%, p<0.05). These results reveal that the increase in MPF activity via a caffeine‐treatment creates a more suitable condition for nuclear reprogramming after SCNT.

This study aimed to improve the level of satisfaction and service quality of a forest experience education program for vulnerable social groups by administering a survey problems of the program and suggested improvements. 428 participants in the program. Reliability analysis of the survey yielded values of, Cronbach’s α value was higher than 0.8 for all items, indicating very high reliability. Importance-performance analysis (IPA), a method of measuring and comparing the degree of satisfaction, found two factors: “first need for correction” which included “diversity of programs” and “professionalism of programs”; and “the need for sustained effort” which included “benefits of programs”, “the role of program instructors” and “the interest of programs”. Previously, forest experience education focused on programs involving base expansion and promotion, or quantitative expansion education. Therefore, this study is meaningful in providing basic data on forest experience education for vulnerable social groups.