Light-emitting diodes are used in applications as diverse as aviation lighting, automotive lighting, advertising, general lighting, and traffic signals. LED panel light source used in an experiment on plant and mushroom growth. To establish the optimum artificial light illumination method for Pleurotus eryngii in closed mushroom factory system, the effects of red LED light quality on growth was investigated. The humidity was maintained more than 90% and temperature was 15~16℃, and CO2 density was 500ppm. The fresh weightand height were higher under red LED light (650nm, 73.1 Pmol/m 2s) comparing with control (fluorescent lamp, 10~20 lux). β-aminoisobutyric acid and total phenol content were higher under red LED light (650nm, 73.1 Pmol/m2s).