For the regeneration of diesel particulate filters (DPF) using non-thermal plasma (NTP), both cost-effectiveness and regeneration efficiency should be raised. This study compared and contrasted the physicochemical characteristics of carbon black and engine particulate matter (PM). After carbon black was put into the DPF, an experimental setup for the oxidation of PM using NTP was created. The findings showed that carbon black and PM samples had comparable oxidation traits, micronanostructures, and C/O elemental ratios. O3, the main active species in NTP, was susceptible to heat breakdown, and the rate of decomposition of O3 increases with increasing temperature. The removal effectiveness of carbon black first improved and subsequently declined with an increase in the NTP injection flow rate during offline DPF regeneration using NTP at room temperature. A relatively high carbon black removal efficiency of 85.1% was achieved at an NTP injection flow rate of 30 L/min.