본 시험은 논에서 벼 대체 여름 사료작물인 수수류를 논 재배하여 생육특성과 수량에서 우수한 품종을 선발할 목적으로 2008년부터 2009년까지 2년간에 걸쳐 충남 천안 농가 포장에서 수행하였다. 도입품종인 수수류 10품종 중에서 ‘Sordan79’는 습해에 비교적 강하고 2차 재생이 잘되며 당도가 높고 병해와 충해에 강한 생육 특성을 보였고 수량 측면에서도 생초수량이 ha 당 92,492 kg, 건물수량이 21,090kg으로 가장 우수한 품종이었다. 따라서 본 연구결과를 토대로 볼 때 중부지역에서 벼 대체 사료작물인 수수류를 논에 재배 할때는 배수가 양호한 논의 선정이 필요하고 수수류 품종 중에서는 ‘Sordan79’를 재배하는 것이 적응성이나 생산성 측면에서 가장 유리하다고 할 수 있다.

This experiment was carried out to know adaptability and forage production and quality of corn and corn + soybean intercropping for silage at paddy field in Chungnam province. growth, forage production and quality of silage corn was higher in case of corn and soybean intercropping than that of corn single planting at paddy field. The height of stem of Kwangpyongok and Sword bean intercropping was somewhat lower than Kwangpyongok single planting and higher at ear height and stem diameter. Fresh yield of Kwangpyongok and Sword bean intercropping was the highest as 45.8 tons per ha among corn monoculture, corn + soybean intercropping. The dry yield of Kwangpyongok and Sword bean intercropping at paddy field was the highest as 20.5 tons per ha among corn monoculture and corn + soybean intercropping. The result of this study showed that 'Kwangpyongok and Sword bean intercropping' had good forage productivity and quality of silage compared with corn monoculture, corn and common bean intercropping.

This study was carried out to find out the effect of establishment or renovation of organic pasture by hoof and tooth cultivation with Korean native goat. This study was conducted from August, 2005 to the end of growing season in 2008 at test field of RDA in Cheonan. Experiment design composed with 4 treatment : Standard(Control, T1), Grazing after application cattle manure(T2), Application cattle manure after grazing(T3), Application cattle manure at half of grazing(T4).

This study was conducted on both Grassland & Forage Research Center, NIAS, Cheonan(Central area) and Sancheong(southem area) from 2005 through 2008, in order to evaluate the effect of various cultivation methods on productivity of organic com silage. Treatments included five cultivation methods(conventional, normal organic, strip seeding, early seeding, late seeding) using a com silage(P3156)-crimson clover(Contea) double crop system. Crimson clover was used for either cover crop or green manure in this experiment.

Plant height of 'Composite 28' was 89㎝ and heading date was on 4 May with green leaf color and showed high yield among three composite lines. A visual score of winter field survival of 'Composite 28' was 1.0. 1st dry matter yield of 'Composite 28' in Cheonan was 8.64ton per ㏊ compared to introduced variety 'Amba'(7.20ton/㏊). Heading date of 'Composite 28' was 7 days earlier than check variety, 'Amba'.

To get good productivity and high feed value of forage, we develop to new Italian ryegrass (Lolium multiflorum Lam.) 'Kogreen'.variety. In Suwon, heading date of 'Kogreen' was on May 3 but in Yonchun was on 8 May that was 2 day later than that of 'Florida 80' and dry matter yield is 12.7ton/㏊, and had a good cold tolerance.

In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 ㎎ 1?¹ 2,4-D, 0.5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. The highest regeneration rate was obtained in MS medium containing 1 ㎎ 1?¹ 2,4-D, 5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 1 ㎎ 1?¹ thiamine-HCl, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5% to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species.

In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of a total of seven cultivars were infected and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation frequency and the expression of the GUS gene were investigated. The highest transformation frequency (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the examined cultivars ranged from 0.4% to 6.7%. GUS histochemical assays, PCR, and southern analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of Italian ryegrass. Thus, optimization of transformation frequency and selection of a suitable cultivar of Italian ryegrass may improve molecular breeding of this species.

In the present study, we have used an annealing-control-primer (ACP)-based differentially display RT-PCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes-such as SnRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes-may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.

The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using whole crop barely (WCB)-whole crop rice (WCR) double cropping system for 3 years. Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total DMY of forages under WCB and WCR in treatment of CF significantly decreased as compared with that of treatment of CCM 150%. However, NDF, ADF and CP content of WCB and WCR were hardly influenced by CCM application.

The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using Italian ryegrass-WCR double cropping system for 3 years (2006~2008). Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total day matter yield (DMY) of Italian ryegrass and WCR in treatment of CCM 150% and CCM 200% significantly decreased as compared with that of treatment of chemical fertilizer (CF). However, NDF, AND and CP content of Italian rye grass and WCR were hardly influenced by CCM application.

The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using rye-whole crop rice (WCR) double cropping system for 3 years. Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total DMY of of rye-WCR in treatment of CCM 150% significantly decreased as compared with that of treatment of CF and CCM 200%. However, NDF, ADF and CP content of rye and WCR were hardly influenced by CCM application.

Managing the fatty acid composition of grazing ruminant diets could lead to meat and milk products t㏊t have higher conjugated linoleic acid (CLA) concentrations, but forage fatty acid dynamics must be more fully understood for a range of forages before grazing systems can be specified. The fatty acid profiles of 23 different forages, including grasses, legumes and wild forage plants were determined. Linolenic(C18:3, 41.1~58.6% of fatty acid), linoleic(C18:2, 10.1~27.4% of fatty acid), and palmitic(C16:0, 9.8~18.0% of fatty acid) acids were the most abundant fatty acids in all species, together representing ~96% of the fatty acids present. Concentrations of fatty acids declined as plants developed, but the fraction contribution of each fatty acid to total fatty acids remained relatively stable over time.

In this study, we conducted to select the promising crops for both uses in the bioethanol and forage production in Korea. The result indicated t㏊t Natsukaje (guinea grass), Gwangpyeongok (corn), Jumbo (sorghum×sudangrass hybrid), SS405 (sorghum×sorghum hybrid), Millex32 (pearl millet), Jeju barnyard grass), Alamo (switch grass) and Selection75 (klein grass) showed the production of biomass from the highest to the lowest in order. However, the order of the production of quality forage was, from the highest to the lowest, Natsukaje (guinea grass), Jumbo (sorghum×sudangrass hybrid), SS405 (sorghum×sorghum hybrid), Gwangpyeongok (corn), Millex32 (pearl millet), Selection75 (klein grass), Jeju (barnyard grass), and Alamo (switch grass). We concluded the Natsukaje (guinea grass) was the best bioethanol crop, and also the Natsukaje (guinea grass) was the best for forage production.

This experiment was conducted to evaluate forage yield and feed value of warm season grass in subtropical area. The species having the fastest heading date was bermudagrass 'ecotype' (24th April) while the heading date of bahia grass 'Argentine' was the latest as 13th August. Plant height and dry matter of switch grass was the highest. Dry matter yield of switch grass was usually greater t㏊n the other entries in Jeju and Wando. Bermudagrass cultivars had higher CP concentration t㏊n the other entries. NDF and ADF concentration in switchgrass was higher t㏊n in bermudgarss and bahiagrass cultivars.

Although whole crop barley are now widely grown as a silage crop in Korea, but silage quality of the whole crop barley produced from farmer's fields have not been published. Therefore, this experiment was conducted to evaluate forage quality of whole crop barley which was participated in Korean quality contest in 2008. These data were classified by region, forage production, added inoculants, planting date and harvest date. Difference on lactic acid of barley silage was detected in the region (p＜0.05), however, there no significant differences in other chemical composition. The moisture and lactic acid were significant differences in dry matter yield of barley silage. There is all the difference between silage added inoculants and control. Differences between planting dates in ash and crude protein (CP) were detected in barley silage (p＜0.05). From comparison within harvest date, lactic acid ㏊d significant differences among barley silage. Differences in forage quality were observed among whole crop barley for silage. Therefore, nutritional quality as well as lactic acid is important in silage quality contest of whole crop barley.

This experiment was conducted to evaluate the effect of seeding rate on the quality and yield of whole crop rice variety, "Nokyang". Whole crop rice variety, "Nokyong", was direct seeded at 25 April. The seeding rate were four level(30, 60, 90, and 120㎏/㏊). There was not found significantly difference plant height and dry matter(DM) content among seeding rate. The average DM content was 44.1%. Dry matter yield of 30㎏/㏊ seeding rate plot was decreased compared to others treatment. The CP yield of 60㎏/㏊ seeding rate plot was the highest among treatments. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content have not been affected in relation to seeding rate. The content of TDN(total digestible nutrient) increased with increased seeding rate until 90㎏/㏊ seeding rate. The highest fresh and DM yield showed at 120㎏/㏊ seeding rate. But there was not found significantly difference among 60, 90 and 120㎏/㏊ seeding rate. Although high seeding rate increase the DM yield, 60㎏/㏊ seeding rate will be recommendable as proper seeding rate for whole crop rice.