본 연구는 항균 활성을 지니는 식물추출물을 찾고 화장품에서 방부제로서 적용하기 위해 진행되었다. 디스크 확산법(disk diffusion method)을 통해 3가지 식물 추출물, 함박꽃나무(천녀목란, Magnolia sieboldii), 오배자(Rhus chinensis), 메타세콰이어(Metasequioa glyptostroboides)가 항균활성을 지니고 있음을 확인하였다. 세 물질의 최소저해농도 (MIC)를 측정한 결과 메타세콰이어는 0.3 ∼ 0.35 %, 함박꽃나무는 0.35 ∼ 0.4 % 농도에서 곰팡이의 생장을 억제하고 오배자는 0.45 ∼ 0.5 % 농도에서 세균의 생장을 억제함을 확인하였다. 또한 추출물 내의 항균 활성을 지니는 성분을 분리하여 분석한 결과 메타세콰이어에서 분리한 caryophyllene oxide와 caryophyllene, 함박꽃나무에서 분리한 costunolide와 dehydrocostus lactone, 오배자에서 분리한 ethyl gallate, ethyl-3-gallate 등이 항균활성을 지닌 물질임을 확인하였다. O/W 에멀션 제형에서 식물 추출물을 넣고 방부력을 확인한 결과 혼합사용 시 세균과 곰팡이 모두에 대한 방부효과가 있음을 확인하였다. 따라서 함박꽃나무, 오배자, 메타세콰이어 추출물의 혼합물은 기존의 화학 방부제를 대체할 천연 방부제로서 화장품에서 응용할 수 있을 것으로 기대된다.

The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination (R2) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values (r2=0.97, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids (84.06 mg g-1), whereas IT188096 had a very low content (29.92 mg g-1).

Specialty barley extracts were prepared and investigated for its antioxidant activity and biological activity. Hunter L* values of the Iksan 86 extracts had higher than that of the Iksan 87 and Zasoojeongchal extracts. The extraction yields of Iksan 86, Iksan 87, and Zasoojeongchal was 8.08, 6.62, and 7.30%, respectively. The contents of total phenolic compounds of the Iksan 86, Iksan 87, and Zasoojeongchal extracts were 16.24, 15.51, and 13.95 GAE mg/g of sample, respectively. The DPPH radical scavenging activity of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 50.00, 33.27, and 7.56% at a 500 ppm, respectively. The samples showed an inhibition of xanthin oxidase. ACE inhibition effect of specialty barley extracts, Iksan 86, Iksan 87, and Zasoojeongchal, was 39.81, 41.06, and 27.78%, respectively. Tyrosinase inhibition rates (%) of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 26.21, 24.57, and 20.00%, respectively. Results indicated that specialty barley extracts possesses various biological activities including antioxidative capacity, xanthin oxidase inhibition activity, angiotensin converting enzyme inhibition activity, and tyrosinase inhibition activity.

A new oat (Avena sativa L.) cultivar 'Darkhorse' was developed by Honam Agricultural Research Institute (HARI) m2005. It was released because ofits earlmess, high forage yield potential and adaptability for late summer sowing. It was devel-oped from the a

A new oat (Avena sativa L.) cultivar, "Highspeed" was developed by Honam Agricultural Research Institute (HARI) in2005. It was released because of its earliness, high forage yield potential, and good adaptability for late summer sowing. "High-speed" was s

A population ofrecombinant inbred lines derived from a cross between Ilpumbyeo, a blast-susceptibleyopowca cultivarofhigh eating-quality and high yield potential, and GL33, a blast-resistance/apowca weedy rice was used to identifv QTLs affect-ing importan

“Wonchubyeo” is a new japonica rice (Oryza sativatute (KAERI) and was released in 2004. In order to select the mutants of Chucheongbyeo with god traits, the seds were ira-diated with 250 Gy gama ray (83.3 rad/min) emited from 60Co in a radiation facility

“Woncheongbyeo” is a new japonica rice (Oryza sativa L.) cultivar developed by mutation techniques from Chu-cheongbyeo. Chucheongbyeo seeds were irradiated with 300 Gy gama ray (83.3 rad/min) emitted from 60Co at a radiation facil-ity of the Korea Atomic

With 3 diferent recurrent parents (RPs) (‘Suwon 345’, ‘Iri 390’ and ‘Milyang 95’) and 3 diferent donor parents (‘Dae-cheongbyeo’, ‘RantaiEmas’ and ‘Chugoku 69’ for Xa1, Xa2 and Xa3, respectively), 9 near isogenic lines (NILs) were developedand subjected t

In the absence of exogeneous nitrogen supply, evaluation of a symbiosis effectiveness of Bradyrhizobium japonicum USDA 110 in a supernodulating soybean mutant, SS2-2, its wild type, Sinpaldalkong 2, and control genotype, Jangyeobkong, was conducted in this study. Nodules in SS2-2 were initially white and similar to its wild type, Sinpaldalkong 2. At the late stage, the wild type nodules became dark pinkish by maturation, by contrast, mature nodules in SS2-2 remained light green to pinkish, indicating a lack of leghemoglobin. Tap root length was short in nodulated symbiotic SS2-2 than that of its wild type and the control genotype. Nodulated root length and nodule density on root length were significantly increased by B. japonicum inoculation, but no significant increase was observed on root length and percentage of nodulation to total root length. Regardless of Bradyrhizobium inoculation, SS2-2 showed higher nodule dry weight and higher acetylene reduction activity (ARA) when compared with its wild type and the control genotype. Inoculation of B. japonicum leaded the increase of ARA in 47 days after planting (DAP), in part because of nodule development. Supernodulating mutant, SS2-2, less responded to B. japonicum induction in terms of nitrogen fixation and nodulation characteristics than its wild type. Thus, interaction of supernodulating soybean mutant with Bradyrhizobium had less symbiotically associated response than normal nodulating soybean.