Mediterranean fruit fly, Ceratitis capitata, is one of the most important quarantine pest worldwide. Fruit flies cause serious damage on orchard, and also can cause quarantine problem to fruit exportation. Recently, because of global warming, there is threats of fruit fly invasion to Korea, especially to Jeju island. So there should be a anticipative research about fruit fly control. Many physical quarantine treatment methods, like heat treatment and low-temperature treatment, are developed to control quarantine pests. Recent researches indicate that low-temperature treatment shows good efficacy on several quarantine pests without phytotoxicity. In this trial, we’ve applied the low-temperature treatment on citrus at laboratory and pilot scale containers to validate efficacy and phytotoxicity. Egg and larvae stage of C. capitata was completely controlled after 5 days and 8 days low-temperature treatment at 0℃ respectively, and there was no phytotoxicity on citrus. These results indicate that the low-temperature treatment can be applied to control fruit fly on citrus fruit.

Since methyl bromide(MB) has been designated as ozone deplete substance(ODS) by Montreal protocol (1989), many MBalternative fumigants like ethyl formate and phosphine gas were developed and commercialized. Ethyl formate(EF) is an effectiveMB alternative fumigant to control insect pests on imported fresh commodities. It is important to develop a proper evaporationmethod to apply EF on large scale fumigation because EF has a high boiling point(54℃). Recently, concurrent treatment ofethyl formate and nitrogen(EF+N2 treatment) has been newly developed. At preliminary test, EF+N2 treatment showed goodevaporation rate and showed high efficacy on quarantine pests without phytotoxicity on fresh commodities. In this trial, we’veapplied the EF+N2 treatment on imported orange and lemon at pilot scale (0.5m3, 10m3) containers and commercial scale containerto validate efficacy and phytotoxicity. 70g/m3 of EF liquid was applied on orange and lemon by EF+N2 treatment method,and showed 100% mortality on tested insect pest(Tetranychus urticae) without phytotoxicity at 0.5m3 and 10m3 scale fumigationtrials, respectively. At commercial scale trial, EF+N2 treatment also showed 100% efficacy on T. urticae and there was nophytotoxic symptoms on imported orange and lemon fruit. These results indicate that the newly developed EF+N2 treatmenthas a potential as a MB alternative, and can be applied on imported fresh commodities for quarantine and pre-shipment purpose.

Methyl bromide (MB) has been banned by Montreal Protocol due to ozone depletion in developed countries since 2005 but uses for quarantine & pre-shipment (QPS) remains exemption. Current MB alternatives such as phosphine gas, ethyl formate has been showing their potential in terms of their no phytotoxic damages to target perishable commodities post fumigation as well as their efficacy at low temperature. We evaluated phosphine (PH3) gas as MB alternatives welsh onion, carrot, and lettuce fumigation. on sensitivity test, larvae of Aphis gossypii were most tolerant to PH3 among the all stage of A. gossypii, Tetranychus urticae and Plutella xylostella. The LC99 and LCT99 value of mixture gas for adult of A. gossypii was 1.79 mg/L (24hr, 5℃) and 33.56 mg h/L(5℃), respectively. On confirmation trials scheduled in 28m3 container for 24hr at 5℃, all stages of T. urticae was completely controlled in 2g/㎥ of phosphine gas, but A. gossypii and M. persicae were not completely controled. No phytotoxic damage was observed in vegetables.