Kiwifruit (Actinidia chinensis Planch.) is an economically important horticultural crop. This study reports a new pathogen of brown leaf spot disease in kiwifruit. Brown leaf spot symptoms were observed on the leaves of cv. Redvita in Jeju, South Korea. The leaf spot diseases appeared as extensive, angular, brown, yellow, white or fused color spots on the leaves. Fungi were isolated and identified as Fusarium tricinctum (Corda) Sacc. based on their morphological and molecular characteristics. Pathogenicity tests were conducted with mycelium and the conidia inoculation. The pathogen was re-isolated from the artificially developed symptoms, and identified as F. tricinctum as Koch’s postulates were confirmed. To our knowledge, this is the first report of the brown leaf spot caused by F. tricinctum on kiwifruit.