본 연구는 농업유전자원센터에 보존되어있는 우리나라 원산재래종 팥 유전자원 164점을 대상으로 농업기초형질을 조사하고, 팥 근연종을 포함하여 180점을 대상으로 분자마커를 이용하여 우리나라 재래종 팥 유전자원의 유전적 다양성을 분석한결과는 다음과 같다. 1. 기초 특성조사 된 자원의 개화일수는 최소 44일에서 최대 83일, 평균 67.1일이었으며 성숙일수는 최소 27일, 최대 42일, 평균 34.2일 이었고 생육일수는 최소 83일, 최대 118일, 평균 101.2이었다. 조사된 자원들은 북한원산자원들이 남한원산자원들보다 다소 일찍 개화하나 늦게성숙하는 경향을 보였다. 2. 대비품종인 충주팥의 개화일수는 65일, 성숙일수는 42일, 생육일수는 107일로 대비품종에 비해 우리 재래종 팥 자원의 평균개화일수는 약 2일 늦은 반면 성숙일수는 약 8일정도 빨랐고, 생육일수는 약 6일정도 빨라 평가 대상재래종자원은 대비품종인 충주팥에 비해 조숙종이 많은것으로 조사되었다. 3. 본 시험에 이용된 우리나라 재래종 자원은 일본의 기상생태형에 배치해본 결과 비교적 조생종에 속하는 것으로분석되었다. 4. 10개의 SSR 마커를 이용하여 다양성을 분석한 결과 PIC0.70을 얻었으며, 집단별로 분석한 결과 다양성은 야생종집단이 가장 다양성이 높고 남한원산 자원이 가장 낮은것으로 조사되었다.

Glutenin is the major factor responsible for the unique viscoelastic dough characterisitcs of wheat flour, which determine mixing and bread baking performance(X.Shan et al, 2007). And early maturity is one of the most important cultural characteristic in Korea because of its winter cropping system. This study is to reveal the genetic properties of Asian wheat landrace collection originated from 6 separate regions such as Korea, China, Japan, Afganistan, Iran, Pakistan, Caucasus, and Middle East. Using germplasms maintained in National Agrobiodiversity Center, RDA, Korea, the variations in morphological character and HMW glutenin subunit composition were investigated. In this study, Glu-A1c(null), Glu-B1b(7+8) and Glu-D1a(2+12) alleles are the most frequent in Asian landrace wheats. When it comes to unique composition, Glu-B1aj(8) and Glu-D1q(2+11) subunits are only in Afghanistan wheat. And Glu-B1k(22), Glu-D1l(12), Glu-D1m(10) subunits are only in accessions from Pakistan, Korea, and China, respectively. The accessions from Iran and Caucasus have the highest PIC value(0.57), which shows wheat origin region has high genetic diversity. Grouping by UPGMA anlysis of combination of Glu-1 allele, most accessions from Afghanistan, Korea, and Japan were in the same group despite of geological distance. Contrasively, many germplasms originated from China, Caucasus, and Middle East were in the other same group. The evaluation of bread baking quality by Glu-1 scoring system, 34 accessions are perfect 10. 16 samples from China and 1 Afghanistan among them were also matured before early June, suitable to Korean cropping system. Especially, 3 accessions(K151847, K151865, K151962) had extremely early maturity, ripened before late May. These genetic resources having good gluten quality and early maturity are expected to be used for Korea wheat breeding system.

This study aimed to evaluate 105 tomato accessions conserved in National Agrobiodiversity Center regarding their resistance to Ralstonia solanacearum, a soil-borne vascular bacterium that causes lethal wilt diseases of a wide range of crops worldwide. All the accessions are Solanum lycopericum var. lycopersicum including cultivar or breeding lines. At the four leaf stage, the seedlings were inoculated by drenching the soil with the bacterial suspension concentrated of 108 CFU/ml. Plant roots were wounded before inoculation by cutting with the knife. Seven accessions including IT 32899 were rated as resistant, while other 98 accessions were rated as susceptible. IT 32899 scored 0.1 of disease rate and 0.7 of disease index. The selected accessions will be used as a material to reveal the mechanism of wilt tolerance and to identify the host gene involved in defense response.

The genus Rubus belongs to the Rosaceae family and is comprised of 600-800 species distributed worldwide. Understanding the genetic relationships and genetic structure in Rubus species is important for enabling efficient management, conservation, characterization and utilization of the species. However, as a minor crop, genetic research foundation was limited to explore genetic diversity and relationships in Rubus species. The present study shows the results of application SSR markers that were developed from SSR-enriched libraries of the one Rubus species (Rubus coreanus Mique.) in our previous study. We used 34 polymorphic microsatellite markers to analysis of genetic diversity within the Rubus species, including redraspberry, blackraspberry, blackberry and mountainberry. All the 34 SSR primers pairs produced 483 polymorphic and reproducible amplification fragments. The largest number of alleles per primer pair was confirmed at GB-RC-167, GB-RC-100, GB-RC-076 and GB-RC-245, which contained 26, 25, 23 and 21, respectively. An average value of polymorphic information contents (PIC) were 0.74 with a range of 0.36 to 0.92. Population structure and phylogenetic analyses showed that all Rubus species formed three largely distinct clusters, which were confirmed by principal coordinate analysis (PCoA). We obtained the results that the developed SSR markers showed a substantial degree of genetic diversity in the various Rubus species distributed in Korea.