Mushroom have long been valued as highly nutritious and tasty foods in many societies throughout the world. Itis known for biological activities including anti-inflammatory and anti-oxidative potential. However little is known about anti-cancer property. In this study, we investigated the anti-prostate cancer activity of mushrooms. For that, eight kinds ofmushrooms such as, T. matsutake, S. crispa, G. lucidum-US, G. lucidum-AS, C. cardinalis-BR, G. frondosa, P. linteus, U.esculenta were extracted with hot water. Among them, three kinds of mushrooms including T. matsutake, G. lucidum-US andC. militaris-BR extracts inhibited prostate specific antigen (PSA) expression in prostate cancer cell, LNCaP. These resultsdemonstrate that some of mushrooms inhibited PSA expression suggesting that the mushrooms might be a candidate for thetreatment of prostate cancer.