쥐오줌풀 부정근으로부터 valepotriates와 valerenic acids 함량을 증진시키고자 다양한 elicitor를 처리하였다. 쥐오줌풀 부정근의 생장은 YE 처리구를 제외한 모든 처리구에서 대조구에 비해 감소하였다. 그러나 valepotriates와 valerenic acids 생산은 모든 처리구에서 대조구에 비해 증가하였으며 특히, valepotriates는 100 μM MeJA 처리구에서 12.56 ± 0.78 mg/l, valerenic acids는 1 g/l YE 처리구에서 10.63 ± 1.1 mg/l 로 가장 높았으며, 이는 대조구에 비해 1.6배와 1.8배의 생산성증가를 가져왔다.

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

인삼 부정근의 생장과 ginsenosides 함량에 미치는 elicitor의 영향을 조사한 결과 chitosan처리구의 생장량은 10 mg/L에서 가장 양호하였으며, ginsenosides의 함량은 5 mg/L처리농도에서 14.48 mg/g . DW로 대조구에 비해 약9%의 함량증대를 가져왔다. 세포내에서 이차대사계를 활성화시키는 중간 신호전달 물질(signal transducer)로 알려진 jasmonic acid를 인삼 부정근 배양에 처리한 결과 부정근의 생장량은 10 uM 처리구에서 ginsenosides함량이 가장 양호하였으나, 인삼 부정근의 생장은 다소 억제되었다.

When the leaves, roots and stem segments of seedling of Polygonatum odoratum were cultured on Murashige and Skoog medium with 2.0mg/l BAP, stem segments were the most efficient explants for adventitious shoot inductino. To observe the efficient combination of growth regulators on the adventitious shoot formation , stem segments were cultured on MS medium with various kinds of cytokinins (BAP, kinetin, zeatin). From this experiment, cytokinin treatement was prerequisite for theadventitious shoot formatino,especially BAP was the most effective. Auxin (NAA or IBA) in combination with cyotokinin highly enhanced the adventitious shoot formation. Twenty five percents of explants produced the adventitious shoots on medium with 2.0mg/l BAP solely, while 83% of explants produced the adventitious shoots on medium with 2.0mg/l BAP and 0.1mg/l IBA. Root formationform adventitious shoot was promoted after transfer to 1/2 MS medium supplemented with 0.1mg/l IBA and 0.5mg/l zeatin, thereafter the plantlets with shoots and roots were cultured on 1/2MS medium lacking growth regulators. When the stem segments were cultured to MS medium with 1.0mg/l 2,4 NAA and IBA , yellow and nodulous cali were formed from the stem segments which were developed into adventitious roots. These roots were actively grew after transferred to MS liquid medium lacking growth regulators.