Background : This study was carried out to provide basic data for in-situ conservation of the rare Glehnia littoralis in Korean Midwest. Methods and Results : In Korean Midwest, The average number of plants with Glehnia littora lis were 7 taxa in per plot, and Sinduri was the most abundant in 8 taxa among the surveyed areas. The total number of plants that appeared together were 16 taxa including Glehnia littor alis, the plants were Artemisia capillaris, Asparagus schoberioides, Calystegia soldanella, Care x kobomugi, Carex pumila, Elymus mollis, Glehnia littoralis, Imperata cylindrica var. koenigii, Ischaemum anthephoroides, Ixeris repens, Lathyrus japonica, Miscanthus sinensis, Oenothera bi ennis, Rosa rugosa, Vitex rotundifolia and Zoysia macrostachya. Among them, Carex kobomug i (46.3%), Elymus mollis (15.9%), and Imperata cylindrica var. koenigii (11.7%) were the pla nts with an average coverage of more than 10%. The plants that appeared in all 4 sites in th e survey area were Carex kobomugi and Carex pumila. Conclusions : Rare medicinal plant of Glehnia littoralis was required to be in-situ conservatio n with accompanying plants of coastal sand dunes.

Collected germplasms of five representative dandelion species (Taraxacum ohwianum, T. platycarpum, T. platypecidum, T. officinale, and T. coreanum) were 104 lines from different habitates in Korea and China. Their genetic diversity was analyzed by genomic fingerprinting method using amplified fragment length polymorphism (AFLP). AFLP results of 6 primer combinations were revealed 1,176 total DNA fragments and 523 polymorphic bands with a 44.4% ratio of polymorphism. On the basis of similarity coefficient analysis by unweight pair group method with arithmetic averages (UPGMA), 104 dandelion germplasm lines were ranged from 0.64 to 0.99 and clustered distinct five group depending on the species. Furthermore, a principal coordinate analysis (PCA) by the application of multi-variate analysis indicated significantly greater differences among species than geographical origins.

활용가치가 높은 부존식물자원인 갈대의 기내 번식을 통한 배양체계를 확립하고 재분화 식물체들의 유전적 다양성을 검토한 결과, 성숙종자 유래의 캘러스를 통한 기내 식물체 재분화는 N6배지에서 MS배지보다 양호하였고, 0.25~0.5 mg/L의 BA를 포함한 N6배지에서 가장 높았다. ISSR 마커를 이용하여 재분화 식물체의 유전적 안정성을 분석한 결과, 검출된 총 94 유전좌중 유전적 다형성은 17%였고, 평균 유전자다양도 값(h)은 0.03, BA 5 mg/L를 포함한 N6배지에서 0.008, NAA 0.1 mg/L와 kinetin 2 mg/L를 포함한 MS 배지에서 0.040으로 나타났다. 이것은 재분화된 갈대식물체 개체간에 유전적으로 구조가 매우 단순하고 균일하며, 유전적 다양성 진단에 ISSR 마커가 효과적임을 시사한다.