Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of (30μM)SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration(30μM) of SNP enhanced it. Although the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone metabolism and bone pathophysiology.

버섯유래 생리활성물질을 탐색하고자, 젖버섯아재비 자실체로부터 각종 컬럼크로마토그래피 및 HPLC 등 기법에 의하여 4종의 azulene계 화합물을 순수히 분리정제 하였다. 분리된 화합물은 각종 물리화학적 특성 및 분광학적 분석자료에 의하여 1-formyl-4-methyl-7-isopropyl azulene (1), lactaroviolin (2), 4-methyl-7-isopropyl-azulene-1-carboxylic acid (3) 및 1-formyl-4-methyl-7-(1-hydroxy-1-methylethyl) azulene (4)로 동정되었다. 이들 화합물의 인터페론 감마 생성에 미치는 영향을 조사하였다. 화합물 1과 4는 자연살해세포주(NK92 cell)에서 인터페론 감마 생성을 농도 의존적으로 억제하였으며, 400 μM농도에서 각각 101.3 %와 92.7 %, 100 μM농도에서 각각 11.9 %와 24.1 %의 높은 저해활성을 보였으며, 화합물 2와 3은 400 μM농도에서 45.9 %와 18.0 %의 다소 낮은 저해활성을 나타내었다.

To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects of the ethanol extracts of Rubus coreanus Miq. (ERC) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis factor-α (TNF-α), and Interferon-γ (IFN-γ) production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased IFN-γ and TNF-α production. Consistent with these results, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanol extracts of ERC in a dose-dependent manner. These results suggest that ERC may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.