As a result of analyzing bioactive compounds according to the maturity stage of strawberry fruits, there was no difference in callistephine content according to the extraction solvent and tended to increase as the maturity stage progressed. There was a difference in accumulation patterns between cultivars with different fruit colors, but the callistephin content of the red strawberry, Seolhyang, was high, while the pink strawberry, Manyeonsel, tended to be low. The extraction efficiency of cyanidine-3-glucoide was higher when water was used as the extraction solvent than ethanol, which was the highest in the green stages in both cultivars and tended to decrease as the maturity stage progressed. The (+)-catechin content according to the maturity stage showed the highest content in the green stages overall, and tended to decrease as the maturity stage progressed. The extraction efficiency of (+)-catechin was good in ethanol, and the (+)-catechin content according to the maturity stage was generally high in the green stages and tended to decrease as the maturity stage progressed. The catechin content in green stages of Manyeonsel was 205.3 mg per 100 g of dry weight, which was very high compared to 115.6 mg in the Seolhyang. The extraction efficiency of epi-catechin was better in water than in ethanol, and the content of epi-catechin tended to be higher in Seolhyang than in Manyeonsel. When extracted with water, the epi-catechin content of Seolhyang increased 16.0-fold from 15.7 mg per 100g dry weight in green stages to 250.3 mg in mature stages, and 3.1-fold from 50.1 mg to 154.2 mg in Manyeonsel. The content of quercetin-3-glucoside tended to be higher when ethanol was used as an extraction solvent than water, and it was 12.2 and 15.7 mg per 100 g of dry weight in Seolhyang and Manyeonsel, respectively, in the highest-content green stages. Quercetin had a higher extraction efficiency from ethanol than water, which tended to be slightly higher, at 2.5 mg in the mature stages of Seolhyang, while at 3.2 mg in Manyeonsel. The extraction efficiency of chlorogenic acid was higher in water than in ethanol, and the content was highest in the green stages and tended to decrease as maturation progressed. When extracted with water, the content in the green stages of Seolhyang and Manyeonsel decreased by 45.3 and 33.9%, from 14.8 and 18.9 mg, respectively, to 8.1 and 12.5 mg during the mature stages, respectively. The content of ellagic acid tended to be higher when water was used as an extraction solvent than ethanol, but there was no difference in content according to maturity stages in the two cultivars. The cinchonine was more efficient in extraction from water than ethanol and tended to increase as the maturation stage progressed in both varieties, the highest at 148.6 mg per 100 g of dry weight in the mature stages of the Manyeonsel.

최근 흑토마토는 기능성 토마토로써 소비자에게 각광을 받고 있으며 다양한 품종이 개발되고 있다. 흑토마토 형 질 결정 유전자를 기반으로 흑토마토 판별용 분자표지 셋트를 개발하였으며, 이를 국내 시판 토마토 품종에 적용 시켜본 결과, 대부분의 흑토마토를 정확하게 판별해 낼 수 있음을 확인할 수 있었다. 토마토 반신위조병 (Verticillium wilt)은 Verticillium dahliae와 Verticillium alboatrum에 의한 곰팡이병으로 전형적인 토양전염성 병해이 다. 토마토 황화 잎말림 바이러스(TYLCV; Tomato yellow leaf curl virus)는 담배가루이를 매개로 전염되며, 최근 기 후온난화와 더불어 미국, 남미, 유럽, 동남아시아, 일본, 중국 등지로 확산되고 있어 다양한 TYLCV에 대한 안정적 저항성을 지닌 품종의 육성이 요구된다. 토마토 반신위조병에 대한 저항성 유전자인 Ve1은 extracellular leucine-rich repeat (eLRR) receptor-like protein으로써 cell surface receptor를, TYLCV 저항성 Ty-1은 RNA-dependent RNA polymerase 를 각기 암호화하고 있음이 기존에 확인된 바 있다. Ve1와 Ty-1에 대한 연관 분자표지는 각기 개발 되어 분자육종에 적극 활용 중에 있으나, 본 연구에서는 유전자 염기서열 내의 다형성에 기반을 둔 functional marker의 개발을 진행하였다. 저항성 Ve1과 이병성 ve1의 염기서열 상의 다형성 중, 이병성 ve1에서 premature stop codon을형성하여 저항성 여부를 직접적으로 결정하는 다형성을 기반으로 cleaved amplified polymorphic sequence (CAPS) 분자표지를 개발하였다. 저항성 Ty-1과 이병성 ty-1의 염기서열 상의 7개의 SNP를 비교분석하여, 단백질 서열 상의 차이를 유도하는 다형성을 기반으로 CAPS 분자표지로 제작하였다. TYLCV 저항성 중 열성유전양상을 보인다고 알려져 있는 ty5저항성에 대해서도 유전자 기반 분자표지를 제작하였다. 본 연구에서 개발된 흑토마토, Ve1, Ty-1, ty5 에 대한 기능성 분자표지는 과색판별 및 병저항성 육종의 효율 및 정확도 향상에 크게 기여할 것으로 기대된다.