본 연구는 생물학적 보전지수(IBI, Index of Biotic Integrity) 평가와 이화학적 수질 분석, 물리적 서식지 평가(QHEI, Qualitative habitate evaluation index)를 이용하여 하천 건강성을 평가하고자 남천의 10개 지점을 대상으로 2006년 8월과 2006년 11월, 2007년 2월, 2007년 4월까지 총 4회에 걸쳐 부착규조 조사를 실시하였다. IBI, QHEI값을 산정하기 위하여 USEPA(1999) 가 제시한 항목 중 우리나라의 환경적 특성에 맞게 수정 보안하여 사용하여 평가하였다. 남천의 하천 생태 건강도 평가 결과, IBI 등급은 '보통', QHEI등급은 '열악', BOD값에 의거한 이화학적 수질등급은 II등급 기준인 '약간좋음'으로 나타나 총체적인 하천생태계 건강도는 '보통'상태로 나타났다. 이는 수 환경에 대한 교란은 적고 생물들이 서식하기에 비교적 양호한 수질임을 시사한다. 정점별로는 St. 6과 St. 7, St. 8, St. 9는 물리적 서식지 지수가 가장 '열악'한 상태로 대부분이 하천의 흐름을 방해하는 교각이나 인공보가 곳곳에 설치되어 있으며, 하상구조가 대부분 단순화되어 있어 수생태계 심각한 교란이 예상되는 정점이다. 또한 St. 10은 전체적으로 서식 환경은 좋으나 생활하수 유입과 함께 하수종말처리장으로부터 배출되는 영양염으로 인한 수생태계의 교란을 초래할 것으로 예상되므로 추후 영양염 유입에 대한 관리가 필요한 것으로 판단된다.

Background: Mahonia Nepalensis DC. (Hoang lien o ro), the specie of the family Berberidaceae, is widely distributed in the high mountainous areas at altitudes 1700 – 1900 m of Vietnam. It is found that the stem of Mahonia nepalensis indicated anti-inflammatory, anti-bacterial and antifungal activities and they are used particularly for the treatment of eczema, psoriasis, and other skin conditions. However, no study on the antioxidant and anti-cancer activities of Mahonia Nepalensis stem has been previously reported. The aim of this study was to evaluate anti-oxidant and anti-cancer activities of Mahonia Nepalensis stem. Methods and Results: The stem pieces of Mahonia Nepalensis were dried and extracted three times with 100% methanol. After that, the extract was suspended in distilled water and then partitioned with n-hexane, ethyl-acetate (EtOAc) and butanol (water saturated BuOH) fractions were then evaporated using a vacuum rotary evaporator. Evaluation of the anti-oxidative activity of Mahonia Nepalensis was carried out using a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-producing system. The results revealed that the ethyl acetate fraction of M. nepalensis possessed higher potential DPPH radical scavenging activity (IC50, 81.88 ± 1.33㎍/㎖) than other fractions as well as BHT (2,6-Di-tert-Butyl-4-methylphenol) (IC50, 250.49 ± 1.60㎍/㎖). The reducing power assay was also investigated and EtOAc fraction showed higher absorbance values than other fractions. At 1.0 mg/ml concentration, EtOAc fraction showed absorbance of 1.72, be higher than Ascorbic acid. Cell viability was evaluated according to the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl tetrazolium Bromide) assay. By MTT assay, all fractions showed a significant reduction in cell viability on COLO 205 (Human colon carcinoma cell) at the highest concentration tested (200㎍/ ㎖) with over 70% decrease in cell viability was obtained, and the highest significantly inhibiting effect occurred in butanol fraction with approximately 90% reduction in cell viability. Conclusion: We demonstrated that Mahonia Nepalensis stem extract has highly potential in anti-cancer activity. Further studies are necessary in order to explore the variety of Mahonia Nepalensis stem to be applied as a valuable natural material.

The objective of this study is to provide pollutant loads delivery ratio for flow duration in Oenam-cheon watershed, which is upstream watershed of Juam Lake. To calculate the delivery ratio by flow duration, rating curves and discharge-loads curves using measured data were established, then Flow Duration Curve(FDC) and pollutant loads delivery ratio curves were constructed. The results show that the delivery ratios for BOD5 for abundant flow(Q95), ordinary flow(Q185), low flow(Q275), and drought flow(Q355) were 23.9, 12.7, 7.1, and 2.9%, respectively. The delivery ratios of same flow regime for T-N were 58.4, 31.2, 17.2 and 7.1%, respectively. While, the delivery ratios T-P were 17.3, 7.5, 3.4, and 1.1% respectively. In general, delivery ratio of high flow condition showed higher value due to the influence of nonpoint source pollution. Based on the study results, generalized equations were developed for delivery ratio and discharge per unit area, which could be used for ungaged watershed with similar pollution sources.