This current research investigated whether job stress mediates the relationship between physical environment and safety behavior of members in organization. Furthermore, we hypothesized that safety climate functions as moderator between physical environment-job stress link. In order to examine the hypotheses, 870 employees in various fields of companies were sampled. Using structural equation modeling(SEM), we conducted moderated mediation model analysis which cab elaborately test the significance of our hypotheses. The results showed that job stress mediated the link between physical environment and safety behavior of members. Moreover, the relationship between physical environment and job stress was moderated by safety climate. The implications and limitations of our study and suggestions for future research were discussed.