본 연구는 남한에 자생하는 한국산 복수초를 원예용 경제작물로 개발하고자 수행하였다, 남한의 21개 자생지를 대상으로 분포지역에 따른 개체군의 형질특성을 조사하여 다음과 같이 식물체의개체군 특성에 의해 4가지 유형으로 구분되었다. 강원도의 백두대간을 중심으로 자생하는 복수초군은 개화시기가 가장 빠르고 초장은 15cm이하로 작았으며 꽃받침이 꽃잎에 비해 큰 것이 특징이다 . 화경은 2.73cm 로 다른 복수초들에 비해 소형이다. 중부지방의 광릉 등지에 자생하는 복수초군은 개화시기가 3월 15일경으로 상대적으로 개화시기가 늦다. 화경은 3.67cm로 나타났다. 충청도 및 서해안 등지에 자생하는 복수초군은 개화시기가 빠르고 화경이 6,82cm 로 꽃이 크며 초장이 길어 관상가치가 뛰어나므로 금후 원예금후 원예작물로 개발 가능성이 높다. 제주도에 자생하는 복소초군은 잎과 꽃이 동시에 발달하고 화색은 밝은 황색이며 엽색도 다른 복수초들에 비해 엷은 빛깔의 초록색으로 나타났다. 또한 우리나라에 자생하는 복수초의염색체는 자생지에 관계없이 2n=16으로 조사되었다.

This study aims to evaluate the antitumor effect of Adonis multiflora, one of the plants in the Ranunculaceae, on mice to which hepatoma cells were transplanted and to suggest its possibility as a candidate natural substance to replace antitumor drugs. We performed the MTT assay to assess the extract had a decrease in the growth rate of hepatoma cells depending on concentration. In particular, 100 ㎍/㎖ of the extract showed 40% of growth retardation rate. We assessed the autophagy activity to identify the inhibitory autophagy mechanism of tumor cells in the extract. This proved that the activity increases more as the concentration of the extract is higher. We conducted the Western blot test to confirmed the expression of two proteins LC3 and p62. The expression of p62 was in inverse proportion to the concentration of the extract whereas LC3-Ⅱ increased more as the concentration of the extract was higher. This showed that an increase in the autophagy relies on the conentration of the extract. We performed a test to discover the influence of the extracts on hepatoma cells transplanted to mice. The test proved that the extract triggers a significant decrease in the growth rate of tumor cells. Compared to the start of the test, the size of tumor cells with 50, 100 and 200 ㎎/㎏ of the extract respectively increased by 4, 3.7 and 3.5 times whereas in the controlling group by 6.3 times. The size of tumor cells in benign tumor controlling group increased by 3.1 times. This showed a significant decrease in the growth rate of tumor cells compared to the controlling group. We carried out the experiment of influence of the extract on the expression of two proteins LC3 and p62 in the tumor tissue transplanted into mice. The experiment showed that LC3-II increases more as the concentration of the extract is higher. However, there was a rapid decrease in p62 with 200 ㎎/㎏ of the extract compared to the controlling group. In this study, we proved that the autophagy activity of Adonis multiflora extract inhibits the growth of hepatoma cells by in vitro and in vivo experiments. In conclusion, the inhibitory autophagy mechanism of tumor cells in the extract can be used as a new treatment of antitumor.