This paper is second part on the auditory thresholds and fish behaviors to the underwater sounds for luring of target species at the set-net in the coast of Cheju. In order to obtain the critical ratio of yellow tails(Seriola quinqueradiata) and the emission level of underwater sound for luring of them, we make experiments to measure the auditory threshold of them using conditioning with electric shock. In state that the white noise with 10dB higher sound pressure level than ambient noise is emitted, the auditory thresholds of yellow tails are measured with 100~116.5dB and they are higher than those in state of no emission of white noise by the masking effects of it. Although sound pressure level of background noise go down, the auditory thresholds go up with frequency above than 300Hz.The critical ratio of yellow-tails in frequency of 80Hz, 100Hz, 200Hz, 500Hz, 800Hz are 46dB, 40dB, 50dB, 52dB, 60dB, 70dB respectively. The sound pressure level of which the signal sound is recognized by yellow tails under the ambient noise is above 100dB and the critical ratio of them is above 40dB.

Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng. Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control. Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.