PURPOSES : A methodology for estimating micro-emission factors using vehicle trajectory data collected from navigation and DTG devices and basic emission factors for each vehicle type of the MOtor Vehicle Emission Simulator (MOVES) is presented. The methodology can calculate micro-emissions based on only the traffic volume and average speed for each vehicle type. METHODS : Cluster analysis was performed by accumulating the trajectories of individual vehicles on a specific road section into speed groups in which vehicles drove with the same range of average speed. Then, the micro-emission factors were estimated for each speed group. RESULTS : Using the vehicle trajectory data revealed that the emissions calculated from micro-emission factors estimated by the proposed methodology were similar to the sum of the emissions calculated from the vehicle trajectories for each vehicle. CONCLUSIONS : The micro-emission factor database for each road type and vehicle type proposed in this study should be useful for estimating vehicle emissions on the road. The proposed method can calculate emissions in the same way as the macroscopic analysis method, using the traffic volume, average speed, and link length.