In the previous molecular cloning study from human salivary gland cDNA library novel clones (C75‐014, C76‐022) were known as candidate genes for proline rich proteins by GenBank data base search and RNA in situ hybridization. C75‐014 and C76‐022 genes were characterized as those expressing excretory basic proteins primarily composed of alanine, proline, and leucine residues, mimicking basic proline‐rich proteins (bPRPs) with helical structures and multiple consensus sequences of phosphorylation sites. In the immunohistochemical stainings using polyclonal antisera against each C75‐014 and C76‐022 peptide showed strong reaction in the secretory granules of striated and excretory ducts. And in Western blot for the different salivary specimens relatively distinctive bands appeared at lower molecular weight, ranging about 15‐50 kDa. This study was aimed to identify the molecular characteristics of C75‐014 and C76‐022 proteins, which showed properties of basic proline rich protein. These data suggest that C75‐014 and C76‐022 are candidate genes for proline rich proteins in human salivary gland, which may play a role for protecting and stabilizing the mucosal epithelium against numerous proteolytic damages and stresses.

A patient complaining of severe pain in the right submandibular area showed a huge sialolith in radiogram. During the operation, the submandibular gland was much indurated, and large amount of pus was discharged out at an incision of the salivary gland. The removed salivary gland contained a huge sialolith in the major excretory duct of submandibular gland, which had an intact grayish-white surface in ovoid shape. In the histological examination its excretory ducts were extensively dilated without extravasation of saliva, and the involved salivary gland was almost destroyed by the granulomatous r eaction. Most of a cinar cells were d isappeared and r eplaced by ductal cells filled with exudative materials. The microsections of sialolith showed typical laminar structures of calcification containing amorphous basophilic material in the center, in which a lot of Gram positive and Gram negative microorganisms were found. In the center of sialolith numerous microorganisms were admixed with mucinous materials which were strongly positive for the antibody of mucin-1, and formed multiple colonies. In the periphery of the bacterial colonies proline rich proteins (PRPs) were condensely localized, and followed by the consistent positive reaction of transglutaminase 4 (TGase-4). These data suggest that the sialolith of this study is formed from the primary nidus of bacterial colony aggregated with salivary mucin-1 and PRPs by the crosslinking reaction of TGase-4.

병원균 감염에 의한 식물체내 프롤린과 암모니아의 농도 변화와 그것의 스트레스 생리학적 의미를 구명하기 위하여 Rhizoctonia spp.를 처리 후 라지 펫치에 감염된 잔디의 생육 및 관련 화학적 성분을 감염이 되지 않은 대조구와 비교하였다. 라지 펫치에 감염된 잔디의 뿌리의 건사율은 대조구에 비해 약 30% 증가하였다. 가용성 단백질 농도는 병원균 처리후 6일째 잎의 경우를 제외하고는 라지 펫치 감염에 따른 유의적인 영향이 없었다. 암모니아 농도 역