This study was carried out to determine the biological activity of Acanthopanax sessiliflorum fruit extracts. The phenolic compound contents of the extracts were 21.4 and 15.8 mg/g in hot water and 60% ethanol extracts. The total anti-oxidant activities of the water and the 60% ethanol extracts at a 200 μg/mL phenolic concent ration were at 92.4±0.8 and 89.2±1.1% in terms of the DPPH radical scavenging activity, 98.3±1.1 and 96.5±3.5% in terms of the ABTS radical decolorization, 2.0±0.6 and 1.2±2.8 PF in terms of the anti-oxidant protection factor, and 66.3±0.8 and 61.4±2.3% in terms of the TBARs inhibitory activity. The activities that inhibited the angiotensin-converting enzyme and xanthin oxidase were at 85.1±3.2 and 0% in the water extracts and 59.3±1.5 and 9.5±0.8% in the 60% ethanol extracts at the 200 μg/mL phenolic concentration. The tyrosinase and elastase inhibitory activities were at 56.6±1.8 and 53.1±1.1% in the water extracts and 33.7±2.2 and 22.4±3.1% in the 60% ethanol extracts. The astringent effect of the water and the 60% ethanol extracts were at 50.5±0.9 and 11.5±4.1%.